Ordering Information
- : 23-028M
- : 250 µg
Product Images
Activity was measured by titrating 82ng of IκBα protein in a ubiquitination assay. Data shown for a representative lot.
Purity was assessed by SDS-PAGE and Coomassie blue staining using 3µg of activated IκBα. Confirmed identity as IκBα by mass spectrometry.
IkBa Protein, activated, 250 μg
Description:
IkBa, activated
Trade Name:
Upstate (Millipore)
Qty/Pk:
250 µg
Background Information:
IκBα is a cellular protein that inhibits the transcription factor NF-κB. NF-κB plays a central role in cellular stress and inflammatory responses by controlling cytokine-inducible gene expression and lymphocyte stimulation by antigens. In non-stimulated cells NF-κB is sequestered in the cytoplasm via the inhibitory protein IκBα, which obscures the nuclear localization signal of NF-κB and also blocks its ability to bind DNA. In response to various stimuli, including viral infection, ultraviolet radiation and inflammatory cytokines, IκBα is phosphorylated by the protein kinase complex IKK, which triggers its rapid degradation by ubiquitin mediated proteolysis. The liberated NF-κB then translocates to the nucleus and activates the expression of target genes. Mutations in the gene for IκBα result in the functional impairment of NF-κB which causes a decreased production of proinflammatory cytokines and certain interferons, rendering patients susceptible to infection.
Applications:
250 µg of IκBα. Activated, full length, recombinant human IκBα. N-terminal c-Myc, 6His-tagged. Phosphorylated in vitro using IKKα, active (Item # 14-461). For use in Enzyme Assays. Behaves as a substrate in ubiquitin assays.
Key Applications:
Enzyme Assay
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Species:
Human
Cascade Component:
Substrate
Brand Family:
Upstate
Specific Activity:
For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
Long Description:
N-terminal c-Myc, 6His-tagged, recombinant human IκBα full length, expressed by baculovirus in Sf21 insect cells. Purified using immobilized metal affinity chromatography. Phosphorylated in vitro using IKKα, active (Item # 14-461)
Product Name:
IkBa, activated
Entrez Gene Number:
Alternate Names:
- NF-kappa-B inhibitor alpha
- I-kappa-B-alpha
- Major histocompatibility complex enhancer-binding protein MAD3
Purification Method:
Purified using immobilized metal affinity chromatography.