Ordering Information
- : 23-030M
- : 250 µg
Product Images
Activity was measured by titrating 400ng of SCF Fbw7 complex in a ubiquitination assay. Data shown for a representative lot.
Purity was assessed by SDS-PAGE and Coomassie blue staining using 3µg of active SCFFbw7 complex. Confirmed identity as Fbw7, Skp1, Cul1 and Rbx1 by ma...
SCF-Fbw7 Protein Complex, Active, 250 μg
Description:
SCFFbw7 complex, active
Trade Name:
Upstate (Millipore)
Qty/Pk:
250 µg
Background Information:
The SCF (Skp1-Cul1-F-box protein) complexes represent the largest family of ubiquitin-protein ligases and mediate the ubiquitination of a broad spectrum of regulatory and signalling proteins in diverse cellular pathways. The SCF consists of three invariant components, Skp1, Cul1, and Rbx1 and an interchangeable subunit, an F-box protein which is responsible for recruiting specific substrates to be ubiquitinated by the SCF. SCFFbw7 mediates the ubiquitination and subsequent proteasomal degradation of target proteins involved in cell division, growth and differentiation, including Cyclin E1, c-Myc, Notch and c-Jun. Mutations have been found in the Fbw7 subunit of SCFFbw7 in various types of human cancers, therefore Fbw7 is considered to be a potent tumour suppressor.
Applications:
250 µg of SCFFbw7 complex. Active complex of four (4) full length, recombinant human enzymes: Fbw7, Skp1, Cul1, and Rbx1. For use in Enzyme Assays. Functions as an E3 ligase in ubiquitination assays.
Key Applications:
Enzyme Assay
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Species:
Human
Cascade Component:
E3
Brand Family:
Upstate
Specific Activity:
For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
Long Description:
Complex of N-terminal GST-tagged, recombinant human Fbw7 full length, N-terminal 6His-tagged, recombinant human Skp1 full length, N-terminal 6His-tagged, recombinant human Cul1 full length and untagged recombinant human Rbx1 full length, co-expressed by baculovirus in Sf21 insect cells. Purified using glutathione sepharose.
Product Name:
SCFFbw7 complex, active
Entrez Gene Number:
Alternate Names:
- SCFFbxw7
- SCFhCdc4
Purification Method:
Purified using glutathione sepharose.