Ordering Information
- : 23-031
- : 10 µg
Product Images
Activity was measured by titrating 208ng of Cyclin E1 complex in a ubiquitination assay. Data shown for a representative lot.
Purity was assessed by SDS-PAGE and Coomassie blue staining using 3µg of Cyclin E1 complex. Confirmed identity as Cyclin E1 and CDK2 by mass spectrome...
Cyclin E1 complex
Description:
Cyclin E1 complex
Trade Name:
Upstate (Millipore)
Qty/Pk:
10 µg
Background Information:
Cyclin E1 functions as a regulator of the cell cycle, promoting the G1 to S phase transition by binding to and activating the cyclin dependent kinase CDK2. The abundance of cyclin E1 fluctuates during a normal cell cycle and is controlled at the level of gene transcription and by ubiquitin dependent proteolysis. Non-complexed cyclin E1 is degraded by the Cul3 pathway, however when cyclin E1 is bound to CDK2 its ubiqutination is dependent on a positive phosphorylation status, which enables its recognition by SCFFbw7. The impact of cyclin E1 quantities on cell cycle regulation is reflected in the elevated levels expressed by many types of human tumours.
Key Applications:
Enzyme Assay
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Species:
Human
Cascade Component:
Substrate
Brand Family:
Upstate
Specific Activity:
For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
Long Description:
Complex of N-terminal c-Myc, 6His-tagged, recombinant human Cyclin E1 full length and untagged, recombinant human CDK2 full length, co-expressed by baculovirus in Sf21 insect cells. Purified using immobilized metal affinity chromatography.
Product Name:
Cyclin E1 complex
Entrez Gene Number:
Alternate Names:
- CDK2/CyclinE
- CDK2/Cyclin E1
Purification Method:
Purified using immobilized metal affinity chromatography.