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Activity was measured by titrating 164ng of MDM2/CK1δ enzyme in a ubiquitination assay. Data shown for a representative lot.
Purity was assessed by SDS-PAGE and Coomassie blue staining using 3µg of active MDM2/CK1δ. Confirmed identity as MDM2 and CK1δ by mass spectrometry.
MDM2/CK1δ Protein, active
Description:
MDM2/CK1δ, active
Trade Name:
Upstate (Millipore)
Qty/Pk:
BULK
Background Information:
MDM2 is an E3 ubiquitin ligase that regulates the turnover of several cellular factors including the p53 tumour suppressor protein and notably MDM2 itself. The suppression of p53 activity by an amplified Mdm2 gene has been observed in many tumours, including soft tissue sarcomas, osteosarcomas and breast tumours.
Applications:
Bulk quantity of MDM2/CK1δ. Active, full length, recombinant human MDM2 (N-terminal GST-tagged) combined in vitro with CK1δ, active (Item # 14-520). For use in Enzyme Assays. Functions as an E3 ligase in ubiquitination assays.
Key Applications:
Enzyme Assay
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Species:
Human
Cascade Component:
E3
Brand Family:
Upstate
Specific Activity:
For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
Long Description:
N-terminal GST-tagged, recombinant human MDM2 full length, expressed in E.coli. Purified using glutathione sepharose followed by gel filtration. Combined in vitro with CK1δ, active (Item # 14-520)
Product Name:
MDM2/CK1δ, active
Entrez Gene Number:
Alternate Names:
- Double minute 2 protein
- p53-binding protein Mdm2
- Hdm2
Purification Method:
Purified using glutathione sepharose followed by gel filtration.