Ordering Information
- : 23-034M
- : 250 µg
Product Images
Activity was measured by titrating 245ng of p53 protein in a ubiquitination assay. Data shown for a representative lot.
Purity was assessed by SDS-PAGE and Coomassie blue staining using 3µg of p53. Confirmed identity as p53 by mass spectrometry.
p53 Protein, 250 μg
Description:
p53
Trade Name:
Upstate (Millipore)
Qty/Pk:
250 µg
Background Information:
p53 is expressed in a wide variety of tissues and plays a major role in preventing tumour development. It is involved in cell cycle regulation, functioning as a trans-activator to negatively regulate cell division by controlling a set of genes required for this process. It acts as a tumour suppressor in many tumour types by stimulating either apoptosis or growth arrest depending on the physiological circumstances and the cell type. Defects in p53 expression have been implicated in several diseases including; choroid plexus papilloma, lung cancer, head/neck squamous cell carcinomas, oesophageal squamous cell carcinoma, Li-Fraumeni syndrome, and hereditary adrenocortical carcinoma.
Applications:
250 µg of p53. Full length, recombinant human p53. N-terminal c-Myc, 6His-tagged. For use in Enzyme Assays. Functions as a substrate in ubiquitination assays.
Key Applications:
Enzyme Assay
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Species:
Human
Cascade Component:
Substrate
Brand Family:
Upstate
Specific Activity:
For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
Long Description:
N-terminal c-Myc, 6His-tagged, recombinant human p53 full length, expressed by baculovirus in Sf21 insect cells. Purified using immobilized metal affinity chromatography.
Product Name:
p53
Entrez Gene Number:
Alternate Names:
- Cellular tumour antigen p53
- Antigen NY-CO-13
- Phosphoprotein p53
- Tumour suppressor p53
- TP53
Purification Method:
Purified using immobilized metal affinity chromatography.