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Ordering Information

  • : 23-036
  • : 10 µg
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  • Product Family Information

UbcH6 Protein, active, 10 μg

Description:
UbcH6, active
Trade Name:
Upstate (Millipore)
Qty/Pk:
10 µg
Background Information:
Ubiquitin-conjugating (E2) enzymes are characterized by the presence of a highly conserved ubiquitin-conjugating domain which accommodates ATP-activated ubiquitin (Ub) via a covalently linked thioester onto its active-site residue. E2 enzymes act via selective protein-protein interactions with the ubiquitin-activating E1 enzyme and ubiquitin ligase E3 enzymes and are able to differentiate effects on downstream substrates, either with a single Ub molecule or a Ub chain. While E3s are involved in substrate selection, E2s are the main determinants for selection of the lysine to construct Ub chains, which thereby directly control the cellular fate of the substrate.
UbcH6 has been shown to interact with a number of diverse E3 ligases, including RNF20/40, Trim32 and WWP2.
Applications:
10 µg of UbcH6. Active, full length, recombinant human UbcH6. N-terminal HA, 6His-tagged. For use in Enzyme Assays. Functions as an E2 enzyme in ubiquitination assays.
Key Applications:
Enzyme Assay
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Species:
Human
Cascade Component:
E2
Brand Family:
Upstate
UniProt Number:
P51965
Long Description:
N-terminal HA, 6His-tagged, recombinant human UbcH6 full length, expressed in E.coli
Product Name:
UbcH6, active
Entrez Gene Number:
NM_003341
Alternate Names:
  • Ubiquitin-conjugating enzyme E2 E1
  • Ubiquitin-protein ligase E1
  • UBE2E1
Purification Method:
Purified using immobilized metal affinity chromatography.