Ordering Information
- : 23-041M
- : 250 µg
Product Images
Activity was measured by titrating 500ng of Cbl enzyme in a ubiquitination assay. Data shown for a representative lot.
Purity was assessed by SDS-PAGE and Coomassie blue staining using 3µg of active c-Cbl. Confirmed identity as c-Cbl by mass spectrometry.
c-Cbl Protein, active, 250 μg
Description:
c-Cbl, active
Trade Name:
Upstate (Millipore)
Qty/Pk:
250 µg
Background Information:
Intracellular signals mediated by receptor tyrosine kinases play a pivotal role in morphogenesis, cell fate determination and pathogenesis. c-Cbl is a ubiquitin ligase (E3) that facilitates the regulation of these pathways, attenuating signalling through ubiquitination and subsequent protein degradation. c-Cbl contains several functional domains, including a tyrosine kinase-binding domain that associates with a number of protein tyrosine kinases in a phosphorylation-dependent manner, a catalytic RING-finger domain that interacts with E2s and is essential for E3 activity and a C-terminal proline rich domain which mediates interactions with SH3 containing proteins. Mutations in the gene for c-Cbl have been implicated in a number of human cancers, particularly acute myeloid leukaemia.
Applications:
250 µg of c-Cbl. Active, full length, recombinant human c-Cbl. N-terminal GST-tagged. For use in Enzyme Assays. Functions as an E3 ligase in ubiquitination assays.
Key Applications:
Enzyme Assay
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Species:
Human
Cascade Component:
E3
Brand Family:
Upstate
Specific Activity:
For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
Long Description:
N-terminal GST-tagged, recombinant human c-Cbl full length, expressed by baculovirus in Sf21 insect cells. Purified using glutathione sepharose.
Product Name:
c-Cbl, active
Entrez Gene Number:
Alternate Names:
- E3 ubiquitin-protein ligase CBL
- Casitas B-lineage lymphoma proto-oncogene
- RING finger protein 55
- Signal transduction protein CBL
- CBL2
- RNF55
Purification Method:
Purified using glutathione sepharose.