Ordering Information
- : 23-042
- : 10 µg
Product Images
Activity was measured by titrating 390ng of Src protein in a ubiquitination assay. Data shown for a representative lot.
Purity was assessed by SDS-PAGE and Coomassie blue staining using 3µg of Src. Confirmed identity as Src by mass spectrometry.
Src Protein, 10 μg
Description:
Src
Trade Name:
Upstate (Millipore)
Qty/Pk:
10 µg
Background Information:
Src is a ubiquitously expressed, non-receptor tyrosine kinase that plays a pivotal role in numerous cellular processes such as proliferation, migration, and transformation. Due to its critical function in regulating signal transduction pathways, Src activity is tightly controlled through reversible phosphorylation and dephosphorylation events and down-regulation by ubiquitin-dependent degradation. Dysfunction of Src, through overexpression or increased activation has profound effects on basic cellular functions. Elevated Src expression and/or activation is evident across a wide range of solid tumour types and has been strongly implicated in the development, maintenance, progression, and metastatic spread of several human cancers, such as prostate, lung, breast, and colorectal cancer.
Applications:
10 µg of Src. Full length, recombinant human Src. N-terminal c-Myc, 6His- tagged. For use in Enzyme Assays. Functions as a substrate in ubiquitination assays.
Key Applications:
Enzyme Assay
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Species:
Human
Cascade Component:
Substrate
Brand Family:
Upstate
Specific Activity:
For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
Long Description:
N-terminal c-Myc, 6His- tagged, recombinant human Src full length, expressed by baculovirus in Sf21 insect cells. Purified using immobilized metal affinity chromatography.
Product Name:
Src
Entrez Gene Number:
Alternate Names:
- Proto-oncogene c-Src
- pp60c-src
- SRC1
Purification Method:
Purified using immobilized metal affinity chromatography.