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Activity was measured by titrating 468ng of Kit protein in a ubiquitination assay. Data shown for a representative lot.
Purity was assessed by SDS-PAGE and Coomassie blue staining using 3µg of Kit. Confirmed identity as Kit by mass spectrometry.
Kit Protein, 250 μg
Description:
Kit
Trade Name:
Upstate (Millipore)
Qty/Pk:
250 µg
Background Information:
Kit belongs to the family of type III receptor tyrosine kinases. It mediates the cellular response to stem cell factor and plays a major role in survival, expansion and differentiation of haematopoietic progenitor cells and reproductive stem cells. The activation of Kit is tightly regulated and uncontrolled Kit activity contributes to the growth of diverse human tumours. Gain-of-function mutations have been described in a number of malignancies, including acute myeloid leukaemia, small cell lung cancer, gastrointestinal stromal tumours and testicular cancer. Negative regulation of Kit signalling has been shown to occur mainly through ubiquitin-mediated internalization and degradation of the receptor.
Applications:
250 µg of Kit. Recombinant human Kit, amino acids 544-end. N-terminal c-Myc, GST-tagged. For use in Enzyme Assays. Functions as a substrate in ubiquitination assays.
Key Applications:
Enzyme Assay
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Species:
Human
Cascade Component:
Substrate
Brand Family:
Upstate
Specific Activity:
For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
Long Description:
N-terminal c-Myc, GST-tagged, recombinant human Kit amino acids 544-end, expressed by baculovirus in Sf21 insect cells. Purified using glutathione sepharose.
Product Name:
Kit
Entrez Gene Number:
Alternate Names:
- Mast/stem cell growth factor receptor
- Proto-oncogene c-Kit
- Tyrosine-protein kinase Kit
- CD_antigen=CD117
Purification Method:
Purified using glutathione sepharose.