Product Images
Activity was measured by titrating 1000ng of VHL enzyme complex in a ubiquitination assay. Data shown for a representative lot.
Purity was assessed by SDS-PAGE and Coomassie blue staining using 3µg of active VHL complex. Confirmed identity as VHL, Elongin C, Elongin B, Cul2 and...
VHL protein complex, active
Description:
VHL complex, active
Trade Name:
Upstate (Millipore)
Qty/Pk:
BULK
Background Information:
The VHL complex is a multi-subunit ubiquitin ligase composed of VHL, Elongin B, Elongin C, Cul2 and Rbx1. The VHL protein serves as the substrate recognition component and is linked by Elongin C to a heterodimeric Cul2/Rbx1 module that functions as a potent activator of the ubiquitination of target proteins by an E2 conjugating enzyme. Elongin B interacts with the complex through Elongin C and appears to stabilize the binding of Elongin C to VHL. The primary function of the VHL complex is to regulate HIF (hypoxia inducible factor) activity by targeting the hydroxylated HIF-1α subunit for ubiquitination and rapid proteasomal degradation under normoxic conditions. It therefore plays an important role in the regulation of hypoxia-inducible genes such as the vascular endothelial growth factor (VEGF) and glucose transport-1 (Glut-1). Mutations in VHL are associated with the inherited von Hippel-Lindau (VHL) cancer syndrome and numerous forms of renal cell carcinoma.
Applications:
Bulk quantity of VHL complex. Active complex of five (5) recombinant human enzymes: VHL amino acids 54-end, full length Elongin C, full length Elongin B, full length Cul2, and full length Rbx1. For use in Enzyme Assays. Functions as an E3 ligase in ubiquitination assays.
Key Applications:
Enzyme Assay
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Species:
Human
Cascade Component:
E3
Brand Family:
Upstate
Specific Activity:
For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
Long Description:
Complex of N-terminal GST-tagged, recombinant human VHL amino acids 54-end, N-terminal GST tagged, recombinant human Elongin C, untagged, recombinant human Elongin B, N-terminal 6His tagged, recombinant human Cul2 and untagged recombinant human Rbx1 co-expressed by baculovirus in Sf21 insect cells. Purified using glutathione sepharose.
Product Name:
VHL complex, active
Entrez Gene Number:
Alternate Names:
- VCB-Cul2 complex
- ECV complex
- CBCVHL
Purification Method:
Purified using glutathione sepharose.