Ordering Information
- : 23-045M
- : 250 µg
Product Images
Activity was measured by titrating 74ng of HIF-1α protein in a ubiquitination assay. Data shown for a representative lot.
HIF-1α Protein, 250 μg
Description:
HIF-1α
Trade Name:
Upstate (Millipore)
Qty/Pk:
250 µg
Background Information:
HIF-1α is the oxygen sensitive subunit of the hypoxia inducible factor HIF-1 which is an essential mediator of the cellular oxygen-signalling pathway. Under hypoxic conditions HIF-1α activates the transcription of over 40 genes, including those that control glucose uptake, metabolism, angiogenesis, erythropoiesis, cell proliferation, and apoptosis. The expression of HIF-1α is commonly increased in a variety of human tumours, and correlates with poor patient prognosis. Under normoxia this protein interacts with the ubiquitin ligase VHL complex, leading to its polyubiquitination and subsequent proteasomal degradation.
Applications:
250 µg of HIF-1α. Recombinant human HIF-1α amino acids 331-609, N-terminal c-Myc, GST-tagged. For use in Enzyme Assays. Functions as a substrate in ubiquitination assays.
Key Applications:
Enzyme Assay
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Species:
Human
Cascade Component:
Substrate
Brand Family:
Upstate
Specific Activity:
For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
Long Description:
N-terminal c-Myc, GST-tagged, recombinant human HIF-1α amino acids 331-609, expressed by baculovirus in Sf21 insect cells. Purified using glutathione sepharose.
Product Name:
HIF-1α
Entrez Gene Number:
Alternate Names:
- Hypoxia-inducible factor 1-alpha
- ARNT-interacting protein
- Basic-helix-loop-helix-PAS protein MOP1
- bHLHe78
- PAS domain-containing protein 8
Purification Method:
Purified using glutathione sepharose.