Ordering Information
- : 23-047M
- : 250 µg
Product Images
Activity was measured by titrating 80ng of active UbcH7 enzyme in a ubiquitination assay. Data shown for a representative lot.
UbcH7 Protein, active, 250 μg
Description:
UbcH7, active
Trade Name:
Upstate (Millipore)
Qty/Pk:
250 µg
Background Information:
Ubiquitin-conjugating (E2) enzymes are characterized by the presence of a highly conserved ubiquitin-conjugating domain, which accommodates ATP-activated ubiquitin (Ub) via a covalently linked thioester on its active-site residue. E2 enzymes act through selective protein-protein interactions with the ubiquitin-activating E1 enzyme and ubiquitin ligase E3 enzymes and are able to differentiate effects on downstream substrates, either with a single Ub molecule or a Ub chain. While E3s are involved in substrate selection, E2s are the main determinants for selection of the lysine to construct Ub chains, which thereby directly control the cellular fate of the substrate.
UbcH7 shows broad specificity for HECT-type E3s but does not function with most RING-containing E3 ubiquitin-protein ligases because it lacks intrinsic E3-independent reactivity with lysine. Despite lacking this lysine reactivity, UbcH7 exhibits activity with the RBR family E3 enzymes, such as Parkin and ARIH1.
UbcH7 shows broad specificity for HECT-type E3s but does not function with most RING-containing E3 ubiquitin-protein ligases because it lacks intrinsic E3-independent reactivity with lysine. Despite lacking this lysine reactivity, UbcH7 exhibits activity with the RBR family E3 enzymes, such as Parkin and ARIH1.
Applications:
250 µg of UbcH7. Active, full length, recombinant human UbcH7. N-terminal HA, 6His-tagged. For use in Enzyme Assays. Functions as an E2 enzyme in ubiquitination assays.
Key Applications:
Enzyme Assay
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Species:
Human
Cascade Component:
E2
Brand Family:
Upstate
Long Description:
N-terminal HA, 6His-tagged, recombinant human UbcH7 full length, expressed in E.coli.
Product Name:
UbcH7, active
Entrez Gene Number:
Alternate Names:
- Ubiquitin-conjugating enzyme E2 L3
- L-UBC
- Ubiquitin carrier protein L3
- Ubiquitin-conjugating enzyme E2-F1
- Ubiquitin-protein ligase L3
- UBE2L3
- UBCE7
Purification Method:
Purified using immobilized metal affinity chromatography.