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Activity was measured by titrating the enzyme in a ubiquitination assay. See Certificate of Analysis for assay details.
Purity was assessed by SDS-PAGE and Coomassie blue staining. Protein identify was confirmed by mass spectrometry.
Parkin Protein, active
Description:
Parkin, active
Trade Name:
Upstate (Millipore)
Qty/Pk:
BULK
Background Information:
Parkin is an E3-ubiquitin ligase belonging to the RBR (RING – In Between RING – RING) family. Mutations in Parkin that result in a loss-of-function are the most frequent cause of familial Parkinson’s disease and are responsible for a large percentage of autosomal recessive juvenile parkinsonism (AR-JP). In addition to its auto-ubiquitination, Parkin can also ubiquitinate various substrates, including the CDK2-interacting protein cyclin E1, the -synuclein-interacting protein synphilin-1, the synaptic vesicle-associated CDC-rel1 and the p38 subunit of aminoacyl-tRNA synthetase/JTV-1. Ubiquitination of substrates occurs in conjunction with the E2 enzymes UbcH7, UbcH8 or UbcH13/Uev1.
Applications:
Bulk quantity of Parkin. Active, full length, human recombinant human Parkin. N-terminal 6His-tagged. For use in Enzyme Assays. Functions as an E3 ligase in ubiquitination assays.
Key Applications:
Enzyme Assay
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Species:
Human
Cascade Component:
E3
Brand Family:
Upstate
Long Description:
N-terminal 6His-tagged, recombinant human Parkin full length, expressed by baculovirus in Sf21 insect cells. Purified using immobilized metal affinity chromatography.
Product Name:
Parkin, active
Entrez Gene Number:
Alternate Names:
- Parkinson juvenile disease protein 2
- PARK2
- PRKN
Purification Method:
Purified using immobilized metal affinity chromatography.