Ordering Information
- : 23-049M
- : 250 µg
Product Images
Activity was measured by titrating the protein in a ubiquitination assay. See Certificate of Analysis for assay details.
Purity was assessed by SDS-PAGE and Coomassie blue staining. Protein identify was confirmed by mass spectrometry.
p38/JTV-1 Protein, 250 μg
Description:
p38/JTV-1
Trade Name:
Upstate (Millipore)
Qty/Pk:
250 µg
Background Information:
p38/JTV-1 is a scaffold required for the assembly and stability of the multi-tRNA synthetase complex, an essential enzyme that ligates specific amino acids to tRNAs before protein synthesis. It plays a pivotal role in the control of cell proliferation and has been shown to interact with FUSE binding protein, stimulating its ubquitination and degradation and leading to the down-regulation of the transcription factor c-Myc which is required for differentiation of functional alveolar type II cells. Genetic disruption of p38/JTV-1 in mice has been observed to cause neonatal lethality.
p38/JTV-1 is ubiquitinated by the E3 ligase Parkin, leading to its proteosomal degradation. It has been shown to accumulate in Parkin-deficient mice and in the brain tissue of patients with hereditary Parkinson’s, suggesting that it may play a role in the pathogenicity of this disease.
p38/JTV-1 is ubiquitinated by the E3 ligase Parkin, leading to its proteosomal degradation. It has been shown to accumulate in Parkin-deficient mice and in the brain tissue of patients with hereditary Parkinson’s, suggesting that it may play a role in the pathogenicity of this disease.
Applications:
250 µg of p38/JTV-1. Full length, recombinant human p38/JTV-1. N-terminal cMyc, GST-tagged. For use in Enzyme Assays. Functions as a substrate in ubiquitination assays.
Key Applications:
Enzyme Assay
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Species:
Human
Cascade Component:
Substrate
Brand Family:
Upstate
Long Description:
N-terminal cMyc, GST-tagged, recombinant human p38/JTV-1 full length, expressed by baculovirus in Sf21 insect cells. Purified using immobilized metal affinity chromatography.
Product Name:
p38/JTV-1
Entrez Gene Number:
Alternate Names:
- Aminoacyl tRNA synthase complex-interacting multifunctional protein 2
- AIMP2
- Multisynthase complex auxiliary component p38
Purification Method:
Purified using immobilized metal affinity chromatography.