Ordering Information
- : 23-050M
- : 250 µg
Product Images
Activity was measured by titrating the enzyme in a ubiquitination assay. See Certificate of Analysis for assay details.
Purity was assessed by SDS-PAGE and Coomassie blue staining. Protein identify was confirmed by mass spectrometry.
CHIP Protein, active, 250 μg
Description:
CHIP, active
Trade Name:
Upstate (Millipore)
Qty/Pk:
250 µg
Background Information:
CHIP is an E3 ubiquitin-protein ligase which targets misfolded chaperone substrates for proteasomal degradation. It contains multiple domains, including a tetratricopeptide repeat (TPR) domain that allows interaction with molecular chaperones, such as Hsp70 and Hsp90 and a U-box domain that confers its E3 ubiquitin ligase function. It has been demonstrated that CHIP preferentially ubiquitinates chaperone-bound substrates, of which there are a diverse range including CFTR, ErbB2 and LRRK2, but also targets Hsp70 itself to the proteasome for degradation when misfolded substrates are not present. This sequential degradation of misfolded substrate and chaperone provides a homeostatic mechanism for regulating chaperone levels to reflect the status of protein folding within the cytoplasm.
Applications:
250 µg of CHIP. Active, full length, recombinant human CHIP. N-terminal 6His-tagged. For use in Enzyme Assays. Functions as an E3 ligase in ubiquitination assays.
Key Applications:
Enzyme Assay
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Species:
Human
Cascade Component:
E3
Brand Family:
Upstate
Long Description:
N-terminal 6His-tagged, recombinant human CHIP full length, expressed in E.coli. Purified using immobilized metal affinity chromatography.
Product Name:
CHIP, active
Entrez Gene Number:
Alternate Names:
- Carboxy terminus of Hsp70-interacting protein
- CLL-associated antigen KW-8
- Antigen NY-CO-7
- STIP1 homology and U box-containing protein 1
- STUB1
Purification Method:
Purified using immobilized metal affinity chromatography.