Ordering Information
- : 23-052M
- : 250 µg
Product Images
Activity was measured by titrating the enzyme in a ubiquitination assay. See Certificate of Analysis for assay details.
Purity was assessed by SDS-PAGE and Coomassie blue staining. Protein identify was confirmed by mass spectrometry.
Hsp70 Protein, 250 μg
Description:
Hsp70
Trade Name:
Upstate (Millipore)
Qty/Pk:
250 µg
Background Information:
Hsp70 proteins are central components of the cellular network of molecular chaperones. They assist in a wide range of processes, including the folding and assembly of newly synthesized proteins, refolding of misfolded and aggregated proteins, membrane translocation of organellar and secretory proteins, and control of the activity of regulatory proteins. The cellular levels of Hsp70 are thought to be at least partially regulated by the E3 ubiquitin ligase CHIP, which also targets a broad range of Hsp70 chaperone substrates for proteosomal degradation. Consistent with its diverse activities, genetic and biochemical studies have implicated Hsp70 in a range of diseases including cancer, neurodegeneration, allograft rejection and infection.
Applications:
250 µg of Hsp70. Full length, recombinant human Hsp70. N-terminal c-Myc, 6His-tagged. For use in Enzyme Assays. Functions as a substrate in ubiquitination assays.
Key Applications:
Enzyme Assay
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Species:
Human
Cascade Component:
Substrate
Brand Family:
Upstate
Long Description:
N-terminal c-Myc, 6His-tagged, recombinant human Hsp70 full length, expressed by baculovirus in Sf21 insect cells. Purified using immobilized metal affinity chromatography.
Product Name:
Hsp70
Entrez Gene Number:
Alternate Names:
- Heat shock 70 kDa protein 1A/1B
- HSPA1A
- HSPA1B
Purification Method:
Purified using immobilized metal affinity chromatography.