Ordering Information
- : 23-054
- : 10 µg
Product Images
Activity was measured by titrating the enzyme in a ubiquitination assay. See Certificate of Analysis for assay details.
Purity was assessed by SDS-PAGE and Coomassie blue staining. Protein identify was confirmed by mass spectrometry.
MuRF1 Protein, active, 10 μg
Description:
MuRF1, active
Trade Name:
Upstate (Millipore)
Qty/Pk:
10 µg
Background Information:
Muscle atrophy is a debilitating and life threatening disorder. It is associated with a number of diseases including cancer, diabetes, chronic obstructive pulmonary disease and AIDS and is a natural consequence of inactivity and aging. MuRF1 is an E3 ubiquitin ligase which has been implicated as a component of atrophy-associated proteolysis. It contains an amino terminal RING finger that mediates ubiquitin transfer along with a zinc finger and two coiled-coil domains in its central region. It is up-regulated in a number of different models of atrophy and interacts with numerous myofibrillar proteins as well as Cardiac Troponin I and Creatine Kinase. Deletion of MuRF1 in mice has been shown to inhibit skeletal muscle atrophy suggesting that it may be an important drug target for the prevention of muscle wasting.
Applications:
10 µg of MuRF1. Active, full length, recombinant human MuRF1. N-terminal GST-tagged. For use in Enzyme Assays. Functions as an E3 ligase in ubiquitination assays.
Key Applications:
Enzyme Assay
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Species:
Human
Cascade Component:
E3
Brand Family:
Upstate
Long Description:
N-terminal GST-tagged, recombinant human MuRF1 full length, expressed in E.coli. Purified using glutathione sepharose.
Product Name:
MuRF1, active
Entrez Gene Number:
Alternate Names:
- E3 ubiquitin-protein ligase TRIM63
- Iris RING finger protein (IRF)
- Muscle-specific RING finger protein 1
- RING finger protein 28 (RNF28)
- Striated muscle RING zinc finger protein (SMRZ)
Purification Method:
Purified using glutathione sepharose.