Ordering Information
Mouse-To-Mouse IHC Detection System Kit, Red color precipitate; For IHC of mouse antibody on mouse tissue sections
Description:
Mouse-To-Mouse IHC Detection System Kit, Red color precipitate; For IHC of mouse antibody on mouse tissue sections
Trade Name:
Chemicon (Millipore)
Product Overview:
CHEMICON's Mouse-to-Mouse IHC Detection kit is designed for staining mouse or rabbit antibodies on mouse or rat tissues. The kit utilized a drastically different new technology to eliminate background problems. The system is biotin-free and more sensitive then other similar systems that are available.
Background Information:
For use with mouse primary antibodies on mouse tissues to prevent the background often associated with mouse-on-mouse immunohistochemistry.
Product also works to detect mouse and rabbit antibodies rat or mouse tissues.
Product also works to detect mouse and rabbit antibodies rat or mouse tissues.
Key Applications:
- Immunohistochemistry
- Immunohistochemistry (Paraffin)
Application Notes:
Immunohistochemistry: (All procedures should be performed at room temperature)
Deparaffinization, rehydration
Block endogenous peroxidase with 3% H2O2 in dd water for 10 minutes.
Perform antigen retrieval or enzyme digestion if necessary.
1.Block with Pre-antibody Blocking solution for 60 minutes. Wash with TBS for 5 minutes, two times.
2.Incubate the mouse or rabbit primary antibody for 30-60 minutes, wash with TBS for 5 minutes, two times. (Incubation times with the primary antibody will need to be determined by the end user.)
3.Block with Post-antibody Blocking solution for 10 minutes. Wash with TBS for 5 minutes, two times
4.Incubate with the ready-to-use Poly-HRP-Anti-Mouse-Rabbit IgG for 10 minutes, wash with TBS for 5 minutes, two times.
5.Apply AEC for 10 minutes. Wash with distilled water.
6.Counterstain with hematoxylin and mount.
NOTE:
1.Blocking for >60 minutes may be required for certain tissues (tonsil, lymph node, etc).
2.DAB can be used to replace AEC as substrate if background is clean and higher sensitivity is needed.
3.AEC staining is organic soluble and should be mounted in an aqueous mounting media.
4.The system is biotin free so that blocking endogenous biotin is not necessary.
Deparaffinization, rehydration
Block endogenous peroxidase with 3% H2O2 in dd water for 10 minutes.
Perform antigen retrieval or enzyme digestion if necessary.
1.Block with Pre-antibody Blocking solution for 60 minutes. Wash with TBS for 5 minutes, two times.
2.Incubate the mouse or rabbit primary antibody for 30-60 minutes, wash with TBS for 5 minutes, two times. (Incubation times with the primary antibody will need to be determined by the end user.)
3.Block with Post-antibody Blocking solution for 10 minutes. Wash with TBS for 5 minutes, two times
4.Incubate with the ready-to-use Poly-HRP-Anti-Mouse-Rabbit IgG for 10 minutes, wash with TBS for 5 minutes, two times.
5.Apply AEC for 10 minutes. Wash with distilled water.
6.Counterstain with hematoxylin and mount.
NOTE:
1.Blocking for >60 minutes may be required for certain tissues (tonsil, lymph node, etc).
2.DAB can be used to replace AEC as substrate if background is clean and higher sensitivity is needed.
3.AEC staining is organic soluble and should be mounted in an aqueous mounting media.
4.The system is biotin free so that blocking endogenous biotin is not necessary.
Species Reactivity:
- Mouse
- Rabbit
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Kit or Assay Type:
- IHC Detection Kits
- Immunohistochemistry Assays
Components:
- Pre-antibody Blocking Solutions (Ready-to-Use) 3ml
- Post-antibody Blocking Solution (Ready-to-Use) 3 ml
- Poly-HRP anti-Mouse/Rabbit IgG (Ready-to-Use) 3 ml
- AEC Substrate (Ready-to-use) 3 ml
Presentation:
Liquid.
The antibodies are isolated from antisera by immunoaffinity chromatography using antigen coupled to agarose beads.
The antibodies are isolated from antisera by immunoaffinity chromatography using antigen coupled to agarose beads.
Storage Conditions:
Maintain product at 2-8°C for up to 12 months.