Kinase Peptide Microarray - Random Threonine Library
Description:
Kinase Peptide Microarray - Random Threonine Library
Trade Name:
Upstate (Millipore)
Qty/Pk:
1 kit
Background Information:
Millipore's Kinase Peptide Microarrays, using JPT technology, offer a very efficient way to detect potential phosphorylation sites in selected kinase substrate peptides derived from kinase substrate proteins. Follwing the incubation with the target kinase in the presence of radioactive ATP, incorporated phosphate can be detected by autoradiography or phosphorimaging. Alternatively, phospho-specific antibody-based fluorescent readout may be used for phospho-peptide detection. Moreover, phospho-specific stains like the Pro-Q-Diamond reagent from Molecular Probes could be used to identify peptidic kinase substrates. This product consists of human phosphorylation sites (13-mers) containing one threonine residue in the center position, displayed on 4 peptide microarrays in three identical subarrays (3 x 384 = 1156 peptide spots per slide).
Key Applications:
Kinase Assay
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Brand Family:
Upstate
Storage Conditions:
Storage of Kinase Peptide Microarray Slides
• Optimal storage conditions for Millipore's JPT kinase peptide microarray slides are in a cool (approx. 4°C / 39°F) and dry environment. Millipore's JPT peptide microarrays are stable for at least 18 month when stored at 4°C (39°F).
• Do not freeze the microarray slides for storage.
Handling of Kinase Peptide Microarray Slides
• Always handle the delicate microarray slides with care.
• Never touch the microarray slide surface.
• Always wear laboratory gloves when handling Millipore's JPT kinase peptide microarrays.
• Please hold the microarrays slides at the end, which carries the engraved data label (frosted area). This label provides for unique identification of the array.
• Please take care when dispensing solutions onto the microarray surface. Make sure not to touch the surface with pipette-tips or dispensers.
• Never whisk the surface of the slide with a cloth.
• Never use other chemicals as described. Inappropriate chemicals may destroy the chemical bonding of the peptides to the glass surface.
• Avoid dust or other particles during each step of the experiment. Dust, particles and resulting scratches will cause artefacts during the final signal readout.
• Please filter all solutions for the washing steps through 2 µm, preferably 0.4 µm particle filters before use.
• Optimal storage conditions for Millipore's JPT kinase peptide microarray slides are in a cool (approx. 4°C / 39°F) and dry environment. Millipore's JPT peptide microarrays are stable for at least 18 month when stored at 4°C (39°F).
• Do not freeze the microarray slides for storage.
Handling of Kinase Peptide Microarray Slides
• Always handle the delicate microarray slides with care.
• Never touch the microarray slide surface.
• Always wear laboratory gloves when handling Millipore's JPT kinase peptide microarrays.
• Please hold the microarrays slides at the end, which carries the engraved data label (frosted area). This label provides for unique identification of the array.
• Please take care when dispensing solutions onto the microarray surface. Make sure not to touch the surface with pipette-tips or dispensers.
• Never whisk the surface of the slide with a cloth.
• Never use other chemicals as described. Inappropriate chemicals may destroy the chemical bonding of the peptides to the glass surface.
• Avoid dust or other particles during each step of the experiment. Dust, particles and resulting scratches will cause artefacts during the final signal readout.
• Please filter all solutions for the washing steps through 2 µm, preferably 0.4 µm particle filters before use.
Protein or Enzyme Type:
Tools for Enzyme Studies
Product Name:
Kinase Peptide Microarray - Random Threonine Library