Ordering Information
Lck KinEASE™ FP-645nm FarRed Assay
Description:
Lck KinEASE™ FP-645nm FarRed Assay
Trade Name:
- KinEASE
- Upstate (Millipore)
Key Applications:
Kinase Assay
Application Notes:
Fluorescence Polarization (FP) is a well known assay format. The KinEASE™ FP assay uses a phosphorylated peptide labeled with a FarRed dye. This phosphorylated tracer has a low molecular weight and thus a low fluorescence polarization value. The phosphorylated tracer binds to a phospho-specific antibody to form a high molecular weight complex with a high polarization value.
In a kinase reaction, a peptide or a protein substrate (non-fluorescently labeled) is phosphorylated by the kinase in the presence of ATP and Magnesium to form a phosphorylated product. This phosphorylated product competes with the tracer for binding to the phospho-specific antibody. As increasing amounts of phosphorylated product are formed from the kinase reaction, there is a reduction in the binding of tracer to antibody resulting in a decrease in the fluorescence polarization value.
In a kinase reaction, a peptide or a protein substrate (non-fluorescently labeled) is phosphorylated by the kinase in the presence of ATP and Magnesium to form a phosphorylated product. This phosphorylated product competes with the tracer for binding to the phospho-specific antibody. As increasing amounts of phosphorylated product are formed from the kinase reaction, there is a reduction in the binding of tracer to antibody resulting in a decrease in the fluorescence polarization value.
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Kit or Assay Type:
- Kinase & Phosphatase Assays
- Kinase Profiling Assays
Components:
- TK Antibody, 20X (Cat.# 35-004)
- TK Substrate 3 (Cat.# 12-554)
- TK Tracer-645nm FarRed, 100X (Cat.# 20-301)
- 10X KinEASE™ Buffer (Cat.# 20-302)
- 1M MgCl2 (Cat.# 20-303)
- Dithiothreitol, 1M (Cat.# 20-265)
- 10mM ATP (Cat.# 20-306)
- 0.5M EDTA, pH 7.2 (Cat.# 20-307)
- 10X Detection Buffer (Cat.# 20-308)
- 0.1M MnCl2 (Cat.# 20-309)
- 384 well KinEASE™ Plates (Cat.# 30-014)
Storage Conditions:
6 months at -20°C
Detection Methods:
Fluoresence Polarization
Gene Symbol:
- LCK
- p56lck
- YT16
- pp58lck
- p56-LCK
- LSK
Analytes Available:
Lck