0 Items| Favorites
> Last Item Added:
0 Items

Ordering Information

  • : 33-037
  • : 50,000 wells
  • View Alternate Pack Size or Formats
  • Product Family Information

PI 3-Kinase HTRF™ Assay; 50,000 wells

Description:
PI 3-Kinase HTRF™ Assay
Trade Name:
Upstate (Millipore)
Qty/Pk:
50,000 wells
Applications:
The PI 3-Kinase HTRF™ Assay is a high-performance assay kit that provides a universal method for testing all Class I PI3-Kinases in a homogeneous format. This pack size has a screening capacity of 50,000 wells.
Key Applications:
Kinase Assay
Species Reactivity:
  • Human
  • Mouse
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Entrez Gene Summary:
Phosphatidylinositol 3-kinase is composed of an 85 kDa regulatory subunit and a 110 kDa catalytic subunit. The protein encoded by this gene represents the catalytic subunit, which uses ATP to phosphorylate PtdIns, PtdIns4P and PtdIns(4,5)P2. This gene has been found to be oncogenic and has been implicated in cervical cancers.
UniProt Summary:
FUNCTION: SwissProt: P42336 # Phosphorylates PtdIns, PtdIns4P and PtdIns(4,5)P2 with a preference for PtdIns(4,5)P2.
SIZE: 1068 amino acids; 124284 Da
SUBUNIT: Heterodimer of a p110 (catalytic) and a p85 (regulatory) subunit. Binds to IRS1 in nuclear extracts. Interacts with RUFY3 (By similarity).
DISEASE: SwissProt: P42336 # Defects in PIK3CA are associated with colorectal cancer (CRC) [MIM:114500]. & Defects in PIK3CA are associated with breast cancer [MIM:114480]. & Defects in PIK3CA are associated with epithelial ovarian cancer (EOC) [MIM:604370].
SIMILARITY: SwissProt: P42336 ## Belongs to the PI3/PI4-kinase family. & Contains 1 C2 domain. & Contains 1 PI3K/PI4K domain.
Brand Family:
Upstate
Storage Conditions:
12 months at -70°C
Target Sub-Family:
PI3K/PI4K
Gene Symbol:
  • PIK3CA
  • MGC142163
  • PI3K
  • MGC142161
  • p110-alpha
Product Name:
PI 3-Kinase HTRF™ Assay
Analytes Available:
PI 3-Kinase
Components:
  • 4X Reaction Buffer
  • PIP2 (Substrate; 1mM stock)
  • Stop Reagent A
  • Stop Reagent B
  • DM A Detection Reagent
  • DM B Detection Reagent
  • DM C Detection Reagent
UniProt Number:
P42336
Detection Methods:
HTRF
Packaging:
Kit capacity: 50,000 wells
Entrez Gene Number:
NM_006218.2