Ordering Information
Product Images
WB: Bim-GST (lane 1), and Bim-GST phosphorylated by JNK1a1/SAPK1c, active (lane 2) were probed with anti-phospho-Bim EL (Ser65) (1μg/ml).
Anti-phospho-Bim EL (Ser65)
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M, R | IP, WB | Rabbit | Purified | Polyclonal Antibody |
UniProt Number:
UniProt Summary
FUNCTION: SwissProt: O43521 # Induces apoptosis. Isoform BimL is more potent than isoform BimEL.
SIZE: 198 amino acids; 22171 Da
SUBUNIT: Forms heterodimers with a number of antiapoptotic Bcl-2 proteins including Mcl-1, Bcl-2, Bcl-X(L), BFL-1, and BHRF1. Does not heterodimerize with proapoptotic proteins such as BAD, BOK, BAX or BAK (By similarity).
SUBCELLULAR LOCATION: Membrane; Peripheral membrane protein. Note=Associated with intracytoplasmic membrane ... see more »
SIZE: 198 amino acids; 22171 Da
SUBUNIT: Forms heterodimers with a number of antiapoptotic Bcl-2 proteins including Mcl-1, Bcl-2, Bcl-X(L), BFL-1, and BHRF1. Does not heterodimerize with proapoptotic proteins such as BAD, BOK, BAX or BAK (By similarity).
SUBCELLULAR LOCATION: Membrane; Peripheral membrane protein. Note=Associated with intracytoplasmic membrane ... see more »
Entrez Gene Number:
Entrez Gene Summary
The protein encoded by this gene belongs to the BCL-2 protein family. BCL-2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. The protein encoded by this gene contains a Bcl-2 homology domain 3 (BH3). It has been shown to interact with other members of the BCL-2 protein family, including BCL2, BCL2L1/BCL-X(L), and MCL1, and to act as an apoptotic activator. The expression of this gene can be induced
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Description:
Anti-phospho-Bim EL (Ser65)
Specificity:
Recognizes phospho-Bim EL (Ser65).
Key Applications:
- Immunoprecipitation
- Western Blotting
Species Reactivity:
- Human
- Mouse
- Rat
Isotype:
IgG
Immunogen:
Peptide corresponding to amino acids 63-71 of human phospho-Bim EL (Ser65).
Modifications:
Phosphorylation
Molecular Weight:
~48 kDa
Control:
Bim protein phosphorylated by active JNK1a1/SAPK11c
Presentation:
Protein A Purified rabbit IgG in a solution of 30% glycerol, 0.07M Tris-glycine, pH 7.4, 0.135M NaCl, and 0.035% sodium azide as a preservative.
Storage Conditions:
Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Gene Symbol:
- BCL2L11
- BimL
- BIM
- BIM-beta7
- BOD
- BAM
- BimEL
- BIM-beta6
- BIM-alpha6
Quality Assurance:
Routinely evaluated by immunoblot on phosphorylated Bim-GST.
Antibody Category:
Apoptosis & Cancer
Antibody Sub-Category:
BCL2 & Inhibition
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Trade Name:
Upstate (Millipore)
Format Code:
Pur
Antibody Type:
Polyclonal Antibody
Format:
Purified
Host:
Rabbit
Purification Method:
Protein A Purified