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画像情報
WB: Bim-GST (lane 1), and Bim-GST phosphorylated by JNK1a1/SAPK1c, active (lane 2) were probed with anti-phospho-Bim EL (Ser65) (1μg/ml).
Anti-phospho-Bim EL (Ser65)
| 交差性 | 主要アプリケーション | 免疫動物 | フォーマット | 抗体タイプ |
|---|---|---|---|---|
| H, M, R | IP, WB | Rabbit | Purified | Polyclonal Antibody |
Description:
Anti-phospho-Bim EL (Ser65)
Trade Name:
Upstate (Millipore)
Specificity:
Recognizes phospho-Bim EL (Ser65).
Molecular Weight:
~48 kDa
Immunogen:
Peptide corresponding to amino acids 63-71 of human phospho-Bim EL (Ser65).
Modifications:
Phosphorylation
Isotype:
IgG
Species Reactivity:
- Human
- Mouse
- Rat
Control:
Bim protein phosphorylated by active JNK1a1/SAPK11c
Quality Assurance:
Routinely evaluated by immunoblot on phosphorylated Bim-GST.
Purification Method:
Protein A purfied
Presentation:
Protein A Purified rabbit IgG in a solution of 0.1M Tris Glycine, 0.15M NaCl, 0.05% Sodium Azide, pH 7.4.
Storage Conditions:
Stable for 1 year at 2-8°C from date of receipt.
UniProt Number:
Entrez Gene Number:
Gene Symbol:
- BCL2L11
- BimL
- BIM
- BIM-beta7
- BOD
- BAM
- BimEL
- BIM-beta6
- BIM-alpha6
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Entrez Gene Summary:
The protein encoded by this gene belongs to the BCL-2 protein family. BCL-2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. The protein encoded by this gene contains a Bcl-2 homology domain 3 (BH3). It has been shown to interact with other members of the BCL-2 protein family, including BCL2, BCL2L1/BCL-X(L), and MCL1, and to act as an apoptotic activator. The expression of this gene can be induced by nerve growth factor (NGF), as well as by the forkhead transcription factor FKHR-L1, which suggests a role of this gene in neuronal and lymphocyte apoptosis. Transgenic studies of the mouse counterpart suggested that this gene functions as an essential initiator of apoptosis in thymocyte-negative selection. Several alternatively spliced transcript variants of this gene have been identified.
Key Applications:
- Immunoprecipitation
- Western Blotting
UniProt Summary:
FUNCTION: SwissProt: O43521 # Induces apoptosis. Isoform BimL is more potent than isoform BimEL.
SIZE: 198 amino acids; 22171 Da
SUBUNIT: Forms heterodimers with a number of antiapoptotic Bcl-2 proteins including Mcl-1, Bcl-2, Bcl-X(L), BFL-1, and BHRF1. Does not heterodimerize with proapoptotic proteins such as BAD, BOK, BAX or BAK (By similarity).
SUBCELLULAR LOCATION: Membrane; Peripheral membrane protein. Note=Associated with intracytoplasmic membranes (By similarity).
DOMAIN: SwissProt: O43521 The BH3 motif is required for Bcl-2 binding and cytotoxicity.
SIMILARITY:
SIZE: 198 amino acids; 22171 Da
SUBUNIT: Forms heterodimers with a number of antiapoptotic Bcl-2 proteins including Mcl-1, Bcl-2, Bcl-X(L), BFL-1, and BHRF1. Does not heterodimerize with proapoptotic proteins such as BAD, BOK, BAX or BAK (By similarity).
SUBCELLULAR LOCATION: Membrane; Peripheral membrane protein. Note=Associated with intracytoplasmic membranes (By similarity).
DOMAIN: SwissProt: O43521 The BH3 motif is required for Bcl-2 binding and cytotoxicity.
SIMILARITY:
Qty/Pk:
200 μg
Brand Family:
Upstate
Format:
Purified
Host:
Rabbit
Product Name:
Anti-phospho-Bim EL (Ser65)
Antibody Type:
Polyclonal Antibody