Guava MultiCaspase Kit
Description:
Guava MultiCaspase Kit
Product Overview:
The Guava® MultiCaspase Assay identifies cell populations in various stages of apoptosis by virtue of a fluorochrome-conjugated caspase inhibitor, sulforhodamine-valyl-alanyl-aspartyl-fluoromethylketone (SR-VAD-FMK).
Non-cytotoxic, cell permeant dye eliminates lysis and permeabilization procedures
Both cell permeable and non-cytotoxic, SR-VAD-FMK can readily cross cell membranes and covalently bind multiple caspase family members that have been activated during apoptosis. Unbound inhibitor is washed away, while covalently coupled reagent is retained inside the cell with the disabled caspases.
Simultaneous staining with the cell-impermeant dye, 7-AAD, serves to assess membrane structural integrity. 7-AAD is excluded from live, healthy cells and early apoptotic cells, but permeates later stage apoptotic and dead cells. For use with all instruments. Click here for more information regarding Instrument Compatibility.
Non-cytotoxic, cell permeant dye eliminates lysis and permeabilization procedures
Both cell permeable and non-cytotoxic, SR-VAD-FMK can readily cross cell membranes and covalently bind multiple caspase family members that have been activated during apoptosis. Unbound inhibitor is washed away, while covalently coupled reagent is retained inside the cell with the disabled caspases.
Simultaneous staining with the cell-impermeant dye, 7-AAD, serves to assess membrane structural integrity. 7-AAD is excluded from live, healthy cells and early apoptotic cells, but permeates later stage apoptotic and dead cells. For use with all instruments. Click here for more information regarding Instrument Compatibility.
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Flow Cytometry Product Type:
Kits & Reagents
Flow Cytometry Kit Category:
Apoptosis
Instrument Compatibility:
- EasyCyte
- EasyCyte Plus
- EasyCyte Mini
- PCA
- PCA-96
Components:
- SR-VAD-FMK
- 7-AAD
- Detailed Protocol