Ordering Information

Catalogue Number : 4500-0150
Qty/Pk : 1 kit

Product Family Information

	Flow Cytometry Kits & Antibodies FlowCellect Kits are EMD Millipore's proprietary multiparameter flow cytometry kits for cellular analysis. We’ve taken the guesswork out of assay development so you can focus on your results. Each kit provides a unique combination of directly conjugated antibodies and/or fluorescent dyes and protein reporters to monitor changes in protein expression and post-translational modification using flow cytometry. The kits also contain complete buffer sets, protocols and pre-defined gate settings. They are fully optimized for "plug-and-play" cellular analysis on Guava EasyCyte and other flow cytometry instruments. In addition, EMD Millipore's line of Milli-Mark fluorescent conjugated antibodies are specifically designed, optimized and validated for flow cytometry, exhibit superior signal-to-noise ratios, and eliminate the need for a secondary antibody. » More…

Guava Express CD3/CD4 Kit

Description:

Trade Name:

Guava

Qty/Pk:

1 kit

Product Overview:

The Guava® Express CD3/CD4 Kit is a two-color direct immunofluorescence reagent kit for enumeration of mature CD4(+) T lymphocytes in human blood. The kit consists of a monoclonal anti-human CD3 antibody conjugated to the tandem dye phycoerythrin (PE)-Cy5 (PECy5), a monoclonal anti-human CD4 antibody conjugated to PE, Guava 1X Lysing Solution to lyse erythrocytes and Guava Fixative to preserve the cells. The CD3 antibody uniquely identifies T cells and recognizes an epitope that is expressed on the epsilon chain of the CD3/T cell antigen receptor (TcR) complex. The CD4 antibody allows the identification of human helper/inducer CD4(+) T cell (HLA Class II reactive) and recognizes a 60,000 Da MW surface antigen. However, the CD4 antigen is also present on monocytes but at much lower density. Monocytes also lack expression of the CD3 molecule.

Key Applications:

Flow Cytometry

Species Reactivity:

Human

Usage Statement:

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Emission:

Sample Format:

Tube
Microplate

Sample Volume, µl:

Flow Cytometry Research Category:

Immunology

Excitation:

Components:

Anti-Human CD4-PE Reagent (1 x 0.1 mL)
Anti-Human CD3-PECy5 Reagent (1 x 0.1 mL)
Guava Antibody Dilution Buffer (1 x 1 mL)
Guava 1X Lysing Solution (1 x 20 mL)
Guava Fixative (1 x 0.5 mL)

Format:

Phycoerythrin/Cy5

Storage Conditions:

2-8 Degrees C

Detection Methods:

Fluorescent

Product Name:

Guava Express CD3/CD4 Kit

Flow Cytometry Market:

Research

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Product Family Information

Flow Cytometry Kits & Antibodies

Guava Express CD3/CD4 Kit

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