Guava EasyCD8 Kit (1000 tests)
Description:
Guava EasyCD8 Kit (1000 tests)
Trade Name:
Guava
Product Overview:
The Guava® EasyCD8™ Reagent Kit is a two-color direct immunofluorescence reagent for enumeration of mature CD8+ suppressor/cytotoxic T lymphocytes in human blood. The reagent consists of an anti-human CD3 antibody conjugated to the tandem dye phycoerythrin (PE)-Cy5(PECy5) and an anti-human CD8 antibody conjugated to PE. The CD3 antibody uniquely identifies T cells and recognizes an epitope that is expressed on the epsilon chain of the CD3/T cell antigen receptor (TcR) complex. The CD8 antibody allows the identification of human suppressor/cytotoxic CD8+ T cell and recognizes an antigen expressed on the 32K Da -subunit of the disulfide linked CD8 biomolecular complex. The cytoplasmic domain of the -subunit of the CD8 antigen is associated with the protein tyrosine kinase p56 1ck. The CD8 antigen is also present on a subset of natural killer (NK) lymphocytes without the co-expression of CD3 molecule. Suppressor/cytotoxic T-lymphocytes (CD3+CD8+) percentages and/or absolute counts may be used to characterize and monitor some forms of immunodeficiency and autoimmune disorders.
Key Applications:
Flow Cytometry
Species Reactivity:
Human
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Emission:
575
Sample Format:
tube
Sample Volume:
200ul
Kit or Assay Type:
Biomarker Detection
Cell Number per Assay:
10ul whole blood
Therapeutic Areas:
Immune Response
Flow Cytometry Research Category:
HIV Monitoring
Excitation:
532
Components:
- Anti-Human CD8-PE Reagent (Part No. 4700-0095, 0.5 mL)
- Anti-Human CD3-PECy5 Reagent (Part No. 4700-0115, 0.5 mL)
- Guava Antibody Dilution Buffer (Part No. 4700-0075, 5 mL)
- Guava 1X Lysing Solution (Part No. 4700-0085, 100 mL)
Format:
Phycoerythrin/Cy5
Storage Conditions:
2-8 Degrees C
Detection Methods:
Fluorescent
Antibody Category:
- Infectious Diseases
- Inflammation & Immunology
Flow Cytometry Software Type:
アッセイ専用モジュール
Flow Cytometry Market:
Clinical
Flow Cytometry Research Subcategory:
CD4 Counting


