Guava Cell Growth Kit for Flow Cytometry
Description:
Guava Cell Growth Kit
Trade Name:
Guava
Qty/Pk:
200 Tests
Product Overview:
The Guava® Cell Growth Assay provides, at the single-cell level, proliferation assessments out to five generations with a simple, non-radioactive assay that is superior to population-based methods such as 3-Thymidine and MTT (or XTT). In addition, the assay offers a broader linear range when compared to colorimetric methods.
Simple, non-radioactive protocol
The assay uses a well-characterized fluorescent dye, carboxyfluorescein diacetate succinimidyl ester (commonly referred to as CFSE), that diffuses freely into cells and is retained within the cell without affecting cellular function. The dye is not transferred to adjacent cells. For each round of cell division, the relative fluorescence intensity of the dye is decreased by half. A dead cell dye, PI, is included to discern the percentage of cells that are dead.
The assay is ideal for primary cultures such as peripheral blood mononuclear cells (PBMCs), activated T- or B-cells, peripheral blood lymphocytes (PBLs), thymocytes, hematopoietic cells, and splenocytes. For use with EasyCyte, EasyCyte Mini, EasyCyte Plus. Click here for more information regarding Instrument Compatibility.
Simple, non-radioactive protocol
The assay uses a well-characterized fluorescent dye, carboxyfluorescein diacetate succinimidyl ester (commonly referred to as CFSE), that diffuses freely into cells and is retained within the cell without affecting cellular function. The dye is not transferred to adjacent cells. For each round of cell division, the relative fluorescence intensity of the dye is decreased by half. A dead cell dye, PI, is included to discern the percentage of cells that are dead.
The assay is ideal for primary cultures such as peripheral blood mononuclear cells (PBMCs), activated T- or B-cells, peripheral blood lymphocytes (PBLs), thymocytes, hematopoietic cells, and splenocytes. For use with EasyCyte, EasyCyte Mini, EasyCyte Plus. Click here for more information regarding Instrument Compatibility.
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Product Name:
Guava Cell Growth Kit
Flow Cytometry Market:
Research
Flow Cytometry Research Subcategory:
Growth