Guava Caspase 3/7 Kit
Description:
Guava Caspase 3/7 Kit
Product Overview:
The Guava® Caspase 3/7 and 8 Assays employ a FLICA (Fluorescent Labeled Inhibitor of Caspases) reagent that specifically identifies active caspase 3 and 7 molecules or caspase 8 molecules.
Non-cytotoxic, cell permeant dyes elimiate lysis and permeabilization procedures
Each FLICA is comprised of three subunits: A caspase-specific amino acid inhibitor containing a recognition sequence for caspase 3/7 (DEVD) or caspase 8 (LETD); a fluoromethylketone moiety (FMK) that forms a covalent linkage with the active enzyme; and a carboxyfluorescein (FAM) reporter. The nuclear DNA stain is also included in the assay to simultaneously evaluate membrane integrity and cell viability. For use with all instruments. Click here for more information regarding Instrument Compatibility.
Non-cytotoxic, cell permeant dyes elimiate lysis and permeabilization procedures
Each FLICA is comprised of three subunits: A caspase-specific amino acid inhibitor containing a recognition sequence for caspase 3/7 (DEVD) or caspase 8 (LETD); a fluoromethylketone moiety (FMK) that forms a covalent linkage with the active enzyme; and a carboxyfluorescein (FAM) reporter. The nuclear DNA stain is also included in the assay to simultaneously evaluate membrane integrity and cell viability. For use with all instruments. Click here for more information regarding Instrument Compatibility.
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Flow Cytometry Product Type:
Kits & Reagents
Flow Cytometry Kit Category:
Apoptosis
Instrument Compatibility:
- EasyCyte
- EasyCyte Plus
- EasyCyte Mini
Components:
- caspase 3/7 (DEVD) or caspase 8 (LETD)
- a fluoromethylketone moiety (FMK) that forms a covalent linkage with the active enzyme
- and a carboxyfluorescein (FAM) reporter. The nuclear DNA stain is also included in the assay to simultaneously evaluate membrane integrity and cell viability.