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Guava RapidQuant Human IgG Kit
Description:
Guava RapidQuant Human IgG Kit
Trade Name:
Guava
Qty/Pk:
1 kit
Product Overview:
The Guava RapidQuant™ Human IgG Kit provides quantitation of human IgG antibodies, typically found in hybridoma supernatants, using a bead-based assay followed by analysis on the Guava EasyCyte™ or EasyCyte™ Plus System. The kit contains IgG Capture Beads (Protein G-bound beads), which bind human antibodies (analyte) through the Fc region. The bound analyte is detected and quantitated using the FITClabeled goat anti-human IgG antibody provided in the kit. A human antibody standard (whole IgG), also provided with the kit, is used at different concentrations to obtain a standard curve. The kit quantitates IgG1, IgG2, IgG3, and IgG4 antibodies in the range of 0.5–20 μg/mL using the single standard provided. This kit works only with intact antibodies that include an Fc region. If the antibody to be tested is an F(ab')2, Fab, sFv, or other variant without an Fc region, this kit should not be used.
Key Applications:
Flow Cytometry
Species Reactivity:
Human
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Emission:
518
Sample Format:
Microplate
Sample Volume, µl:
200
Flow Cytometry Research Category:
Antibody Quantitation
Excitation:
488
Components:
- Guava® IgG Capture Beads (Part No. 4200-0350)
- Guava® Anti-Human IgG FITC (Part No. 4700-1050)
- Guava® Human IgG Standard (Part No. 4100-1110)
- Guava® Bead Buffer 1 (Part No. 4200-0420)
- Guava® Bead Buffer 2 (Part No. 4200-0440)
Format:
FITC
Detection Methods:
Fluorescent
Product Name:
Guava RapidQuant Human IgG Kit
Flow Cytometry Software Type:
Assay Specific Module
Flow Cytometry Market:
- Bioprocess
- Research