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Guava RapidQuant Mouse IgG Kit
Description:
Guava RapidQuant Mouse IgG Kit
Trade Name:
Guava
Qty/Pk:
1 kit
Product Overview:
The Guava RapidQuant™ Mouse IgG Kit provides quantitation of Mouse IgG antibodies, typically found in hybridoma supernatants, using a bead-based assay followed by analysis on the Guava EasyCyte™ or EasyCyte™ Plus System. The kit contains IgG Capture Beads (Protein Gbound beads), which bind mouse antibodies (analyte) through the Fc region. The bound analyte is detected and quantitated using the FITC-labeled goat anti-mouse IgG antibody provided in the kit. A mouse antibody standard (whole IgG), also provided with the kit, is used at different concentrations to obtain a standard curve. The kit quantitates IgG1, IgG2a, and IgG2b antibodies in the range of 2.5-40 μg/ mL using the single standard provided. It also detects IgG3, but at a lower efficiency than the othersubtypes. This kit works only with intact antibodies that include an Fc region. If the antibody to be tested is an F(ab')2, Fab, sFv, or other variant without an Fc region, this kit should not be used.
Key Applications:
Flow Cytometry
Species Reactivity:
Mouse
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Emission:
518
Sample Format:
Microplate
Sample Volume, µl:
200
Flow Cytometry Research Category:
Antibody Quantitation
Excitation:
488
Components:
- Guava® IgG Capture Beads (Part No. 4200-0350)
- Guava® Anti-Mouse IgG FITC (Part No. 4700-1120)
- Guava® Mouse IgG Standard (Part No. 4100-1130)
- Guava® Bead Buffer 1 (Part No. 4200-0420)
- Guava® Bead Buffer 2 (Part No. 4200-0440)
Format:
FITC
Detection Methods:
Fluorescent
Product Name:
Guava RapidQuant Mouse IgG Kit
Flow Cytometry Software Type:
Assay Specific Module
Flow Cytometry Market:
- Bioprocess
- Research