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Guava® Caspase 3/7 SR and Caspase 8 FAM Kit
Description:
Guava® Caspase 3/7 SR and Caspase 8 FAM Kit
Trade Name:
Guava
Qty/Pk:
100 Tests
Product Overview:
The Guava Caspase Assay Suite of fluorescent-based assays for Caspase
3/7, 8, 9 and multi-caspase employ the same protocol making it easier
to perform multiple caspase assays in parallel. Further, Guava caspase
reagents are membrane permeable allowing one to measure intracellular
levels of the enzyme without using harsh lysis methods. The Guava
Caspase Assays employ a FLICA (Fluorescent Labeled Inhibitor of
Caspase) reagent that specifically identifies active caspases. Each FLICA is
comprised of three subunits:
– an active site binding peptide to confer specifi city (DEVD for caspase 3/7,
LETD for caspase 8, LEHD for caspase 9, and VAD for multi-caspase)
– a covalent crosslinking agent (FMK)
– a green (FAM) or orange-red fl uorescent probe (SR): SR is excited by blue
or green lasers (PCA Systems or EasyCyte Systems), FAM by blue lasers
only (EasyCyte Systems)
The Guava Caspase Assays also include the nuclear DNA stain, 7-AAD, to
simultaneously evaluate membrane integrity and cell viability.
3/7, 8, 9 and multi-caspase employ the same protocol making it easier
to perform multiple caspase assays in parallel. Further, Guava caspase
reagents are membrane permeable allowing one to measure intracellular
levels of the enzyme without using harsh lysis methods. The Guava
Caspase Assays employ a FLICA (Fluorescent Labeled Inhibitor of
Caspase) reagent that specifically identifies active caspases. Each FLICA is
comprised of three subunits:
– an active site binding peptide to confer specifi city (DEVD for caspase 3/7,
LETD for caspase 8, LEHD for caspase 9, and VAD for multi-caspase)
– a covalent crosslinking agent (FMK)
– a green (FAM) or orange-red fl uorescent probe (SR): SR is excited by blue
or green lasers (PCA Systems or EasyCyte Systems), FAM by blue lasers
only (EasyCyte Systems)
The Guava Caspase Assays also include the nuclear DNA stain, 7-AAD, to
simultaneously evaluate membrane integrity and cell viability.
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Flow Cytometry Research Category:
Apoptosis & Cancer
Instrument Compatibility:
- EasyCyte
- EasyCyte Plus
- EasyCyte Mini
Components:
- Caspase 3/7 SR and Caspase 8 FAM reagents.
- 7AAD nuclear DNA to simultaneously evaluate membrane integrity and cell viability.
Flow Cytometry Product Type:
Kits & Reagents
Storage Conditions:
2-8°C
Product Name:
Guava® Caspase 3/7 SR and Caspase 8 FAM Kit
Flow Cytometry Software Type:
Assay Specific Module
Flow Cytometry Market:
- Bioprocess
- Research
Flow Cytometry Research Subcategory:
Mid