Ordering Information

Catalogue Number : 46-610
Qty/Pk : 1 kit

Product Family Information

	MILLIPLEX MAP – Human Cell Signaling Portfolio The cell’s ability to respond to its environment is essential for normal development, cellular repair and immunity and is the basis of homeostasis. Errors in these pathways, caused by mutations that may result in constitutive expression or total inhibition of protein transcription, are responsible for such diseases as diabetes, autoimmune disorders and cancer. » More…

MILLIPLEX MAP Phospho p38/SAPK (Thr180/Tyr182) MAPmate - Cell Signaling Single Plex or Multiplex Assay

Description:

MILLIPLEX MAP Phospho p38/SAPK (Thr180/Tyr182) MAPmate

Trade Name:

MILLIPLEX

Qty/Pk:

1 kit

Applications:

This MILLIPLEX® MAP Phospho p38/SAPK (Thr180/Tyr182) MAPmate is a Cell Signaling single plex or multiplex assay. The analyte available for this kit is p38/SAPK2A/B (Thr180/Tyr182).

Key Applications:

Multiplexing

Application Notes:

The MILLIPLEX MAP Phospho p38/SAPK (Thr180/Tyr182) MAPmates pair is used in conjunction with the MILLIPLEX MAP Cell Signaling Buffer and Detection Kit (Catalog # 48-602) to detect the presence of phosphorylated p38/SAPK (Thr180/Tyr182) in cell lysates using the Luminex® 100 IS, 200, or HTS system.

Species Reactivity:

Human

Usage Statement:

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Entrez Gene Summary:

The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation, and development. This kinase is most closely related to p38 MAP kinase, both of which can be activated by proinflammatory cytokines and environmental stress. This kinase is activated through its phosphorylation by MAP kinase kinases (MKKs), preferably by MKK6. Transcription factor ATF2/CREB2 has been shown to be a substrate of this kinase.

UniProt Summary:

FUNCTION: SwissProt: Q15759 # Kinase involved in a signal transduction pathway that is activated by changes in the osmolarity of the extracellular environment, by cytokines, or by environmental stress. Phosphorylates preferentially transcription factor ATF2.
COFACTOR: Magnesium (By similarity).
SIZE: 364 amino acids; 41357 Da
TISSUE SPECIFICITY: Highest levels in the brain and heart. Also expressed in the placenta, lung, liver, skeletal muscle, kidney and pancreas.
DOMAIN:SwissProt: Q15759 The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
PTM: Dually phosphorylated on Thr-180 and Tyr-182, which activates the enzyme (Probable).
SIMILARITY: Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily. & Contains 1 protein kinase domain.

Brand Family:

Upstate

Modifications:

Phosphorylation

Gene Symbol: