MILLIPLEX MAP Phospho JNK/SAPK1 (Thr183/Tyr185) MAPmate

MILLIPLEX MAP Phospho JNK/SAPK1 (Thr183/Tyr185) MAPmate

MILLIPLEX

1 kit

Multiplexing

The MILLIPLEX MAP Phospho JNK/SAPK1 (Thr183/Tyr185) MAPmates pair is used in conjunction with the MILLIPLEX MAP Cell Signaling Buffer and Detection Kit (Catalog # 48-602) to detect the presence of phosphorylated JNK/SAPK1 (Thr183/Tyr185) in cell lysates using the Luminex® 100 IS, 200, or HTS system.

Human

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The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various cell stimuli, and targets specific transcription factors, and thus mediates immediate-early gene expression in response to cell stimuli. The activation of this kinase by tumor-necrosis factor alpha (TNF-alpha) is found to be required for TNF-alpha induced apoptosis. This kinase is also involved in UV radiation induced apoptosis, which is thought to be related to cytochrom c-mediated cell death pathway. Studies of the mouse counterpart of this gene suggested that this kinase play a key role in T cell proliferation, apoptosis and differentiation. Four alternatively spliced transcript variants encoding distinct isoforms have been reported.

FUNCTION: SwissProt: P45983 # JNK1 isoforms display different binding patterns: beta-1 preferentially binds to c-Jun, whereas alpha-1, alpha-2, and beta- 2 have a similar low level of binding to both c-Jun or ATF2. However, there is no correlation between binding and phosphorylation, which is achieved at about the same efficiency by all isoforms.
COFACTOR: Magnesium.
SIZE: 427 amino acids; 48296 Da
SUBUNIT: Binds to at least four scaffolding proteins, MAPK8IP1/JIP-1, MAPK8IP2/JIP-2, MAPK8IP3/JIP-3/JSAP1 and SPAG9/MAPK8IP4/JIP-4. These proteins also bind other components of the JNK signaling pathway. Interacts with TP53 and WWOX. Interacts with JAMP (By similarity).
DOMAIN: SwissProt: P45983 The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
PTM: Dually phosphorylated on Thr-183 and Tyr-185, which activates the enzyme.
SIMILARITY: Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily. & Contains 1 protein kinase domain.

Single Plex

• Anti-JNK/SAPK1 Beads
• Anti-phospho-JNK/SAPK1 (Thr183/ Tyr185), Biotin

Upstate

P45983

Cell Signaling

• Fluorescent
• Luminex xMAP

100 assay points

Phosphorylation

• MAPK8
• PRKM8
• JNK-46
• SAPK1
• JNK1A2
• JNK1
• JNK21B1/2
• JNK

Antibody Arrays

MILLIPLEX MAP Phospho JNK/SAPK1 (Thr183/Tyr185) MAPmate

• NM_002750.2
• NM_139046.1
• NM_139047.1
• NM_139049.1

JNK, JNK/SAPK1 (Thr183/Tyr185)