Phospho-JNK/SAPK1 (Thr183/Tyr185) Beadmates™
Description:
Phospho-JNK/SAPK1 (Thr183/Tyr185) Beadmates™
Trade Name:
MILLIPLEX
Key Applications:
Multiplexing
Application Notes:
Optimal antibody pair for the detection of Phospho-JNK/SAPK1 (Thr183/ Tyr185) using the Beadlyte® Cell Signaling Buffer Kit (Catalogue No. 48-600) and the Luminex® Instrument System (Catalogue No. 40-010) .
Species Reactivity:
Human
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Descriptive Text:
The stress-activated protein kinase 1 family is also referred to as the jun N-terminal kinase family, in light of the substrate preference of these serine/threonine kinases. The SAPK family shares 42-45% identity in the kinase domains with the MAPK family, and the SAPKs are activated by phosphorylation of a threonine and tyrosine by MKK4 and SKK4, just as the MAP kinases are phosphorylated on a threonine and tyrosine by MEK1 and MEK2. By contrast to the mitogen-activated kinases, the stress-activated kinases are only weakly activated by mitogenic stimuli, but potently activated by stress stimuli, such as inflammatory cytokines, ischemia, chemotherapeutic agents and irradiation.
The JNK/SAPK1 kinases, like the other MAPK-like kinases, are thought to phosphorylate multiple substrates and regulate many processes, including proliferation (in some cell types) and apoptosis. The SAPK2/3 family is most widely referred to as the p38 family. These kinases are also activated by stresses, most notably inflammatory cytokines, irradiation, and certain toxins such as anisomycin and arsenite. The activating kinases of SAPK2/3 are SKK2/MEK3 for SAPK2a and 2b, and MKK6 for SAPK3. The targets of the SAPK2/3 family include the MAPKAP kinases 2 and 3/3pK. In addition, SKK4 is related to this family, exhibiting 60% identity, and is activated by MKK6.
The JNK/SAPK1 kinases, like the other MAPK-like kinases, are thought to phosphorylate multiple substrates and regulate many processes, including proliferation (in some cell types) and apoptosis. The SAPK2/3 family is most widely referred to as the p38 family. These kinases are also activated by stresses, most notably inflammatory cytokines, irradiation, and certain toxins such as anisomycin and arsenite. The activating kinases of SAPK2/3 are SKK2/MEK3 for SAPK2a and 2b, and MKK6 for SAPK3. The targets of the SAPK2/3 family include the MAPKAP kinases 2 and 3/3pK. In addition, SKK4 is related to this family, exhibiting 60% identity, and is activated by MKK6.
Therapeutic Areas:
Oncology
Modifications:
Phosphorylation
Packaging:
100 assay points
Components:
- Beadlyte™ Anti-JNK/SAPK1 Beads
- Beadlyte™ Anti-phospho-JNK/SAPK1 (Thr183/ Tyr185), Biotin
Kit Type:
- Cancer Kits
- Cell Signaling Kits
Gene Symbol:
- MAPK8
- PRKM8
- JNK-46
- SAPK1
- JNK1A2
- JNK1
- JNK21B1/2
- JNK
Analytes Available:
JNK
Detection Methods:
Luminex xMAP
Kit or Assay Type:
- Cancer Kits
- Cell Signaling Kits
- Multiplex Assays
Assay Type:
Multiplex Assays