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  • : 46-618
  • : 1 kit
  • Product Family Information

MILLIPLEX MAP Total JNK/SAPK1 MAPmate - Cell Signaling Single Plex or Multiplex Assay

Description:
MILLIPLEX MAP Total JNK/SAPK1 MAPmate
Trade Name:
MILLIPLEX
Qty/Pk:
1 kit
Applications:
This MILLIPLEX® MAP Total JNK/SAPK1 MAPmate is a Cell Signaling single plex or multiplex assay. TThe analyte available for this kit is JNK/SAPK1 (Total).
Key Applications:
Multiplexing
Application Notes:
The MILLIPLEX MAP Total JNK/SAPK1 MAPmates pair is used in conjunction with the MILLIPLEX MAP Cell Signaling Buffer and Detection Kit (Catalog # 48-602) to detect the presence of total JNK/SAPK1 in cell lysates using the Luminex® 100 IS, 200, or HTS system.
Species Reactivity:
Human
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Entrez Gene Summary:
The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various cell stimuli, and targets specific transcription factors, and thus mediates immediate-early gene expression in response to cell stimuli. The activation of this kinase by tumor-necrosis factor alpha (TNF-alpha) is found to be required for TNF-alpha induced apoptosis. This kinase is also involved in UV radiation induced apoptosis, which is thought to be related to cytochrom c-mediated cell death pathway. Studies of the mouse counterpart of this gene suggested that this kinase play a key role in T cell proliferation, apoptosis and differentiation. Four alternatively spliced transcript variants encoding distinct isoforms have been reported.
UniProt Summary:
FUNCTION: SwissProt: P45983 # JNK1 isoforms display different binding patterns: beta-1 preferentially binds to c-Jun, whereas alpha-1, alpha-2, and beta- 2 have a similar low level of binding to both c-Jun or ATF2. However, there is no correlation between binding and phosphorylation, which is achieved at about the same efficiency by all isoforms.
COFACTOR: Magnesium.
SIZE: 427 amino acids; 48296 Da
SUBUNIT: Binds to at least four scaffolding proteins, MAPK8IP1/JIP-1, MAPK8IP2/JIP-2, MAPK8IP3/JIP-3/JSAP1 and SPAG9/MAPK8IP4/JIP-4. These proteins also bind other components of the JNK signaling pathway. Interacts with TP53 and WWOX. Interacts with JAMP (By similarity).
DOMAIN: SwissProt: P45983 The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
PTM: Dually phosphorylated on Thr-183 and Tyr-185, which activates the enzyme.
SIMILARITY: Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily. & Contains 1 protein kinase domain.
Brand Family:
Upstate
Gene Symbol:
  • MAPK8
  • PRKM8
  • JNK-46
  • SAPK1
  • JNK1A2
  • JNK1
  • JNK21B1/2
  • JNK
Product Name:
MILLIPLEX MAP Total JNK/SAPK1 MAPmate
Analytes Available:
JNK, JNK/SAPK1 (Total)
Configuration:
Single Plex
Components:
  • Anti-JNK/SAPK1 Beads
  • Anti-JNK/SAPK1, Biotin
UniProt Number:
P45983
Panel Type:
Cell Signaling
Detection Methods:
  • Fluorescent
  • Luminex xMAP
Packaging:
100 assay points
Entrez Gene Number: