MILLIPLEX MAP Total p21 MAPmate

MILLIPLEX MAP Total p21 MAPmate

MILLIPLEX

1 kit

Multiplexing

The MILLIPLEX MAP Total p21 MAPmates pair is used in conjunction with the MILLIPLEX MAP Cell Signaling Buffer and Detection Kit (Catalog # 48-602) to detect the presence of total p21 in cell lysates using the Luminex® 100™ IS, 200™, or HTS system.

Human

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

This gene encodes a potent cyclin-dependent kinase inhibitor. The encoded protein binds to and inhibits the activity of cyclin-CDK2 or -CDK4 complexes, and thus functions as a regulator of cell cycle progression at G1. The expression of this gene is tightly controlled by the tumor suppressor protein p53, through which this protein mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. This protein can interact with proliferating cell nuclear antigen PCNA), a DNA polymerase accessory factor, and plays a regulatory role in S phase DNA replication and DNA damage repair. This protein was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of CDK2, and may be instrumental in the execution of apoptosis following caspase activation. Two alternatively spliced variants, which encode an identical protein, have been reported

May be the important intermediate by which p53 mediates its role as an inhibitor of cellular proliferation in response to DNA damage. Binds to and inhibits cyclin-dependent kinase activity, preventing phosphorylation of critical cyclin-dependent kinase substrates and blocking cell cycle progression.

Single Plex

• MILLIPLEX MAP Anti- p21 Beads
• MILLIPLEX MAP Anti- p21, biotin
• MILLIPLEX MAP A549 lysate: Camptothecin

Upstate

P38936

Cell Signaling

Recommended storage for kit components is 2 - 8°C

• Luminex xMAP
• Fluorescent

WAF1/Cip1, also known as p21, interacts with cdks associated with cyclins A, D1, D2, D3, and E, to inhibit cdk activity. By contrast with other cdk inhibitors such as p16ink4 and p15ink4B, Cip1 only binds to cyclin/cdk complexes. Expression of Cip1 can block cell cycle progression, alter morphology, and induce differentiation. In addition, Cip1 appears to function as a tumor suppressor as its expression causes malignant cells to accumulate at the G0/G1 boundary. The expression of Cip1 is transcriptionally regulated by p53 and its function is critical for p53-dependent G1 arrest following DNA damage.

MILLIPLEX MAP Total p21 MAPmate

NG_009364

p21(Total)