Ordering Information

Catalogue Number : 46-628
Qty/Pk : 1 kit

Product Family Information

	MILLIPLEX MAP – Human Cell Signaling Portfolio The cell’s ability to respond to its environment is essential for normal development, cellular repair and immunity and is the basis of homeostasis. Errors in these pathways, caused by mutations that may result in constitutive expression or total inhibition of protein transcription, are responsible for such diseases as diabetes, autoimmune disorders and cancer. » More…

MILLIPLEX MAP Total IRS1 MAPmate - Cell Signaling Single Plex or Multiplex Assay

Description:

MILLIPLEX MAP Total IRS1 MAPmate

Trade Name:

MILLIPLEX

Qty/Pk:

1 kit

Applications:

This MILLIPLEX® MAP Total IRS1 MAPmate is a Cell Signaling single plex or multiplex assay. The analyte available for this kit is IRS1 (Total).

Key Applications:

Multiplexing

Application Notes:

The MILLIPLEX MAP Total IRS1 MAPmates pair is used in conjunction with the MILLIPLEX MAP Cell Signaling Buffer and Detection Kit (Catalog # 48-602) to detect the presence of total IRS1 in cell lysates using the Luminex® 100 IS, 200, or HTS system.

Species Reactivity:

Human

Usage Statement:

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Entrez Gene Summary:

no gene summary given

UniProt Summary:

FUNCTION: SwissProt: P35568 # May mediate the control of various cellular processes by insulin. When phosphorylated by the insulin receptor binds specifically to various cellular proteins containing SH2 domains such as phosphatidylinositol 3-kinase p85 subunit or GRB2. Activates phosphatidylinositol 3-kinase when bound to the regulatory p85 subunit (By similarity).
SIZE: 1242 amino acids; 131591 Da
SUBUNIT: Interacts with the NPXY motif of tyrosine-phosphorylated IGF1R and INSR via the PTB domain. Binds to phosphatidylinositol 3-kinase p85 subunit via the phosphorylated YXXM motifs. Binds ROCK1. Binds to UBTF and PIK3CA in nuclear extracts (By similarity). Interacts with SOCS7.
PTM: Serine phosphorylation of IRS1 is a mechanism for insulin resistance. Ser-312 phosphorylation inhibits insulin action through disruption of IRS1 interaction with the insulin receptor (By similarity). & Phosphorylation of Tyr-896 is required for GRB2-binding (By similarity).DISEASE:SwissProt: P35568 # Polymorphisms in IRS1 may be involved in the etiology of non-insulin-dependent diabetes mellitus (NIDDM) [MIM:125853].
SIMILARITY: SwissProt: P35568 ## Contains 1 IRS-type PTB domain. & Contains 1 PH domain.

Brand Family:

Upstate

Storage Conditions:

1 year at 4°C

Gene Symbol: