MILLIPLEX MAP Phospho CREB (Ser133) MAPmate - Cell Signaling Single Plex or Multiplex Assay
Description:
MILLIPLEX MAP Phospho CREB (Ser133) MAPmate
Trade Name:
MILLIPLEX
Qty/Pk:
1 kit
Applications:
This MILLIPLEX® MAP Phospho CREB (Ser133) MAPmate is a Cell Signaling single plex or multiplex assay. The analyte available for this kit is CREB (Ser133).
Key Applications:
Multiplexing
Application Notes:
The MILLIPLEX MAP Phospho CREB (Ser133) MAPmates pair is used in conjunction with the MILLIPLEX MAP Cell Signaling Buffer and Detection Kit (Catalog # 48-602) to detect the presence of phosphorylated CREB (Ser133) in cell lysates using the Luminex® 100 IS, 200, or HTS system.
Species Reactivity:
Human
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Entrez Gene Summary:
This gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. This protein binds as a homodimer to the cAMP-responsive element, an octameric palindrome. The protein is phosphorylated by several protein kinases, and induces transcription of genes in response to hormonal stimulation of the cAMP pathway. Alternate splicing of this gene results in two transcript variants encoding different isoforms.
UniProt Summary:
FUNCTION: SwissProt: P16220 # This protein binds the cAMP response element (CRE), a sequence present in many viral and cellular promoters. CREB stimulates transcription on binding to the CRE.
SIZE: 341 amino acids; 36688 Da
SUBUNIT: Binds DNA as a dimer. Interacts PPRC1 and with HTLV-1 Tax-1.
SUBCELLULAR LOCATION: Nucleus.
PTM: Stimulated by phosphorylation. Phosphorylation of both Ser- 133 and Ser-142 in the SCN regulates the activity of CREB and participates in circadian rhythm generation. Phosphorylation of Ser-133 allows CREBBP and EP300 binding (By similarity). Phosphorylated upon DNA damage, probably by ATM or ATR.
SIMILARITY: SwissProt: P16220 ## Belongs to the bZIP family. & Contains 1 bZIP domain. & Contains 1 KID (kinase-inducible) domain.
SIZE: 341 amino acids; 36688 Da
SUBUNIT: Binds DNA as a dimer. Interacts PPRC1 and with HTLV-1 Tax-1.
SUBCELLULAR LOCATION: Nucleus.
PTM: Stimulated by phosphorylation. Phosphorylation of both Ser- 133 and Ser-142 in the SCN regulates the activity of CREB and participates in circadian rhythm generation. Phosphorylation of Ser-133 allows CREBBP and EP300 binding (By similarity). Phosphorylated upon DNA damage, probably by ATM or ATR.
SIMILARITY: SwissProt: P16220 ## Belongs to the bZIP family. & Contains 1 bZIP domain. & Contains 1 KID (kinase-inducible) domain.
Brand Family:
Upstate
Storage Conditions:
1 year at 4°C
Modifications:
Phosphorylation
Gene Symbol:
- CREB1
- MGC9284
- CREB
Product Name:
MILLIPLEX MAP Phospho CREB (Ser133) MAPmate
Analytes Available:
CREB (Ser133)
Configuration:
Single Plex
Components:
- Anti-CREB Beads
- Anti-Phospho-CREB (Ser133), Biotin
UniProt Number:
Panel Type:
Cell Signaling
Detection Methods:
- Fluorescent
- Luminex xMAP
Packaging:
Kit capacity: 100 Assay Points
Entrez Gene Number: