MILLIPLEX MAP Phospho STAT5A/B (Tyr694/699) MAPmate - Cell Signaling Single Plex or Multiplex Assay
Description:
MILLIPLEX MAP Phospho STAT5A/B (Tyr694/699) MAPmate
Trade Name:
MILLIPLEX
Qty/Pk:
1 kit
Product Overview:
Optimal antibody pair for detection of STAT5A phosphorylated on Tyr694 and STAT5B phosphorylated on Tyr699. To be used in conjunction with the MILLIPLEX MAP Cell Signaling Buffer and Detection Kit (48-602).
Applications:
This MILLIPLEX® MAP Phospho STAT5A/B (Tyr694/699) MAPmate is a Cell Signaling single plex or multiplex assay. The analyte available for this kit is STAT5A/B (Tyr694/699).
Key Applications:
Multiplexing
Application Notes:
The MILLIPLEX MAP Phospho STAT5A/B (Tyr694/699) MAPmates pair is used in conjunction with the MILLIPLEX MAP Cell Signaling Buffer and Detection Kit (Catalog # 48-602) to detect the presence of phosphorylated STAT5A/B (Tyr694/699) in cell lysates using the Luminex® 100 IS, 200, or HTS system.
Species Reactivity:
Human
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Entrez Gene Summary:
The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein mediates the signal transduction triggered by various cell ligands, such as IL2, IL4, CSF1, and different growth hormones. It has been shown to be involved in diverse biological processes, such as TCR signaling, apoptosis, adult mammary gland development, and sexual dimorphism of liver gene expression. This gene was found to fuse to retinoic acid receptor-alpha (RARA) gene in a small subset of acute promyelocytic leukemias (APLL). The dysregulation of the signaling pathways mediated by this protein may be the cause of the APLL.
UniProt Summary:
FUNCTION: SwissProt: P51692 # Carries out a dual function: signal transduction and activation of transcription. Binds to the GAS element and activates PRL-induced transcription.
SIZE: 787 amino acids; 89866 Da
SUBUNIT: Forms a homodimer or a heterodimer with a related family member. Binds NR3C1 (By similarity). Interacts with NCOA1, NMI and SOCS7.
SUBCELLULAR LOCATION: Cytoplasm (By similarity). Nucleus (By similarity). Note=Translocated into the nucleus in response to phosphorylation (By similarity).
PTM: Tyrosine phosphorylated (By similarity).
DISEASE: SwissProt: P51692 # Defects in STAT5B are the cause of Laron type dwarfism II (LTD2) [MIM:245590]; also known as Laron syndrome type II or Laron syndrome due to a post-receptor defect. The phenotypic features are consistent with growth hormone deficiency in the presence of normal to elevated circulating concentrations of growth hormone, and resistance to hexogeneous hormone therapy.
SIMILARITY: SwissProt: P51692 ## Belongs to the transcription factor STAT family. & Contains 1 SH2 domain.
SIZE: 787 amino acids; 89866 Da
SUBUNIT: Forms a homodimer or a heterodimer with a related family member. Binds NR3C1 (By similarity). Interacts with NCOA1, NMI and SOCS7.
SUBCELLULAR LOCATION: Cytoplasm (By similarity). Nucleus (By similarity). Note=Translocated into the nucleus in response to phosphorylation (By similarity).
PTM: Tyrosine phosphorylated (By similarity).
DISEASE: SwissProt: P51692 # Defects in STAT5B are the cause of Laron type dwarfism II (LTD2) [MIM:245590]; also known as Laron syndrome type II or Laron syndrome due to a post-receptor defect. The phenotypic features are consistent with growth hormone deficiency in the presence of normal to elevated circulating concentrations of growth hormone, and resistance to hexogeneous hormone therapy.
SIMILARITY: SwissProt: P51692 ## Belongs to the transcription factor STAT family. & Contains 1 SH2 domain.
Brand Family:
Upstate
Storage Conditions:
1 year at 4°C
Gene Symbol:
- STAT5A
- MGF
- STAT5
Product Name:
MILLIPLEX MAP Phospho STAT5A/B (Tyr694/699) MAPmate
Analytes Available:
STAT5A/B, STAT5A/B (Tyr694/699)
Configuration:
Single Plex
Components:
- Anti-STAT5A/B Beads
- Anti-Phospho-STAT5A/B (Tyr694/699), Biotin
UniProt Number:
Panel Type:
Cell Signaling
Detection Methods:
- Fluorescent
- Luminex xMAP
Packaging:
100 assay points
Entrez Gene Number: