MILLIPLEX MAP Total IκBα MAPmate - Cell Signaling Single Plex or Multiplex Assay

MILLIPLEX MAP Total IκBα MAPmate

MILLIPLEX

1 kit

This MILLIPLEX® MAP Total IκBα MAPmate is a Cell Signaling single plex or multiplex assay. The analyte available for this kit is IκBα (Total).

Multiplexing

The MILLIPLEX MAP Total IκBα MAPmates pair is used in conjunction with the MILLIPLEX MAP Cell Signaling Buffer and Detection Kit (Catalog # 48-602) to detect the presence of total IκBα in cell lysates using the Luminex® 100 IS, 200, or HTS system.

Human

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

NFKB1 (MIM 164011) or NFKB2 (MIM 164012) is bound to REL (MIM 164910), RELA (MIM 164014), or RELB (MIM 604758) to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA or NFKBIB, MIM 604495), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, MIM 600664, or IKBKB, MIM 603258) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime (where H is A, C, or T; R is an A or G purine; and Y is a C or T pyrimidine).[supplied by OMIM]

FUNCTION: SwissProt: P25963 # Inhibits NF-kappa-B by complexing with and trapping it in the cytoplasm. May be involved in regulation of transcriptional responses to NF-kappa-B, including cell adhesion, immune and proinflammatory responses, apoptosis, differentiation and growth. Controlled by sequential serine-phosphorylation, ubiquitination and degradation. Tyrosine-phosphorylation could only lead to dissociation from NF-kappa-B.
SIZE: 317 amino acids; 35609 Da
SUBUNIT: Interacts with p65 (RELA). Interacts with NKIRAS1 and NKIRAS2. Interacts with HBV protein X.
SUBCELLULAR LOCATION: Cytoplasm.
PTM: Phosphorylated; disables inhibition of NF-kappa-B DNA-binding activity. & Ubiquitinated; subsequent to stimulus-dependent phosphorylation on serines, polyubiquitination targets the protein for rapid degradation via the ubiquitin system.
DISEASE: SwissProt: P25963 # Defects in NFKBIA are a cause of autosomal dominant anhidrotic ectodermal dysplasia with immunodeficiency (AD-EDA-ID) [MIM:164008]. Ectodermal dysplasias (EDs) constitute a heterogeneous group of developmental disorders affecting tissues of ectodermal origin. EDs are characterized by abnormal development of two or more ectodermal structures such as hair, teeth, nails and sweat glands, with or without any additional clinical sign. Each combination of clinical features represents a different type of ectodermal dysplasia.
SIMILARITY: SwissProt: P25963 ## Belongs to the NF-kappa-B inhibitor family. & Contains 5 ANK repeats.

Upstate

• NFKBIA
• IKB-alpha
• IKBA
• MAD3
• IkappaBalpha
• I-kappa-B-alpha
• NFKBI
• MAD-3

MILLIPLEX MAP Total IκBα MAPmate

IκBα (Total)

Single Plex

• Anti-IκBα Beads
• Anti-IκBα, Biotin

P25963

Cell Signaling

• Fluorescent
• Luminex xMAP

NM_020529.1