MILLIPLEX MAP Total IGF1R MAPmate - Cell Signaling Single Plex or Multiplex Assay
Description:
MILLIPLEX MAP Total IGF1R MAPmate
Trade Name:
MILLIPLEX
Qty/Pk:
1 kit
Applications:
This MILLIPLEX® MAP Total IGF1R MAPmate is a Cell Signaling single plex or multiplex assay. The analyte available for this kit is IGF-1R (Total).
Key Applications:
Multiplexing
Application Notes:
The MILLIPLEX MAP Cleaved PARP MAPmates pair is used in conjunction with the MILLIPLEX MAP Cell Signaling Buffer and Detection Kit (Catalog # 48-602) to detect the presence of cleaved PARP in cell lysates using the Luminex® 100 IS, 200, or HTS system.
Species Reactivity:
Human
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Entrez Gene Summary:
This receptor binds insulin-like growth factor with a high affinity. It has tyrosine kinase activity. The insulin-like growth factor I receptor plays a critical role in transformation events. Cleavage of the precursor generates alpha and beta subunits. It is highly overexpressed in most malignant tissues where it functions as an anti-apoptotic agent by enhancing cell survival
UniProt Summary:
FUNCTION: This receptor binds insulin-like growth factor 1 (IGF1) with a high affinity and IGF2 with a lower affinity. It has a tyrosine-protein kinase activity, which is necessary for the activation of the IGF1-stimulated downstream signaling cascade.
CATALYTIC ACTIVITY: ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.
ENZYME REGULATION: Autophosphorylation activates the kinase activity.
SUBUNIT: Tetramer of 2 alpha and 2 beta chains linked by disulfide bonds. The alpha chains contribute to the formation of the ligand-binding domain, while the beta chain carries the kinase domain. Interacts with PIK3R1 and with the PTB/PID domains of IRS1 and SHC1 in vitro when autophosphorylated on tyrosine residues.
SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
TISSUE SPECIFICITY: Expressed in a variety of tissues.
PTM: The cytoplasmic domain of the beta subunit is autophosphorylated on tyrosine residues in response to insulin-like growth factor I (IGF I). Phosphorylation of Tyr-980 is required for IRS1- and SHC1-binding.
DISEASE: Defects in IGF1R may be a cause in some cases of resistance to insulin-like growth factor 1 (IGF1 resistance) [MIM:270450]. IGF1 resistance is a gowth deficiency disorder characterized by intrauterine growth retardation and poor postnatal growth accompanied with increased plasma IGF1.
SIMILARITY: Belongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily. Contains 3 fibronectin type-III domains. Contains 1 protein kinase domain.
CATALYTIC ACTIVITY: ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.
ENZYME REGULATION: Autophosphorylation activates the kinase activity.
SUBUNIT: Tetramer of 2 alpha and 2 beta chains linked by disulfide bonds. The alpha chains contribute to the formation of the ligand-binding domain, while the beta chain carries the kinase domain. Interacts with PIK3R1 and with the PTB/PID domains of IRS1 and SHC1 in vitro when autophosphorylated on tyrosine residues.
SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
TISSUE SPECIFICITY: Expressed in a variety of tissues.
PTM: The cytoplasmic domain of the beta subunit is autophosphorylated on tyrosine residues in response to insulin-like growth factor I (IGF I). Phosphorylation of Tyr-980 is required for IRS1- and SHC1-binding.
DISEASE: Defects in IGF1R may be a cause in some cases of resistance to insulin-like growth factor 1 (IGF1 resistance) [MIM:270450]. IGF1 resistance is a gowth deficiency disorder characterized by intrauterine growth retardation and poor postnatal growth accompanied with increased plasma IGF1.
SIMILARITY: Belongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily. Contains 3 fibronectin type-III domains. Contains 1 protein kinase domain.
Brand Family:
Upstate
Storage Conditions:
Store in the dark at 4°C.
Gene Symbol:
- INSR
- CD220
- HHF5
- IR
Product Name:
MILLIPLEX MAP Total IGF1R MAPmate
Specificity:
Recognizes human IGF1R, both phosphorylated and non-phosphorylated forms.
Analytes Available:
CREB, IGF-1R (Total)
Configuration:
Single Plex
Components:
- Anti-IGF1R, Biotin
- Anti-IGF1R Beads
UniProt Number:
Panel Type:
Cell Signaling
Detection Methods:
- Luminex xMAP
- Fluorescent
Packaging:
Kit capacity: 100 Assay Points
Entrez Gene Number: