MILLIPLEX MAP Phospho-ErbB2/HER2 (pan Tyr) MAPmate

MILLIPLEX MAP Phospho-ErbB2/HER2 (pan Tyr) MAPmate

MILLIPLEX

1 kit

Multiplexing

The MILLIPLEX MAP Phospho-ErbB2/HER2 (pan Tyr) MAPmates pair is used in conjunction with the MILLIPLEX MAP Cell Signaling Buffer and Detection Kit (Catalog # 48-602) to detect the presence of phosphorylated ErbB2/HER2 (pan Tyr) in cell lysates using the Luminex® 100™ IS, 200™, or HTS system.

Human

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This gene encodes a member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. This protein has no ligand binding domain of its own and therefore cannot bind growth factors. However, it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signalling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase. Allelic variations at amino acid positions 654 and 655 of isoform a(positions 624 and 625 of isoform b) have been reported, with the most common allele, Ile654/Ile655, shown here. Amplification and/or overexpression of this gene has been reported in numerous cancers, including breast and ovarian tumors. Alternative splicing results in several additional transcript variants, some encoding different isoforms and others that have not been fully characterized.

FUNCTION: Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. Not activated by EGF, TGF-alpha and amphiregulin.
CATALYTIC ACTIVITY: ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.
SUBUNIT: Heterodimer with each of the other ERBB receptors (Potential). Interacts with PRKCABP and PLXNB1. Part of a complex with EGFR and either PIK3C2A or PIK3C2B. May interact with PIK3C2B when phosphorylated on Tyr-1196. Interacts with MEMO when phosphorylated on Tyr-1248. Interacts with MUC1. Stimulation by heregulin (HRG) in breast cancer cell lines induces binding of MUC1 with gamma-catenin.
SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
PTM: Ligand-binding increases phosphorylation on tyrosine residues (By similarity).
POLYMORPHISM: There are fours alleles due to the variations in positions 654 and 655. Allele B1 (Ile-654/Ile-655) has a frequency of 0.782; allele B2 (Ile-654/Val-655) has a frequency of 0.206; allele B3 (Val-654/Val-655) has a frequency of 0.012.
SIMILARITY: Belongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily. Contains 1 protein kinase domain.

Upstate

Recommended storage for kit components is 2 - 8°C

MILLIPLEX MAP Phospho-ErbB2/HER2 (pan Tyr) MAPmate

Recognizes human ErbB2/HER2, both phosphorylated and non-phosphorylated forms.

CREB, ErbB2/HER2 (pan Tyr)

Single Plex

• Anti-ErbB2/HER2, Beads
• Anti-phospho ErbB2/HER2 (pan Tyr), biotin
• A431 Cell Lysate: EGF Stimulated

P04626

Cell Signaling

• Luminex xMAP
• Fluorescent

Kit capacity: 100 Assay Points

NM_001005862