Product Images
Detection of Phospho c-Met/HGFR (pan Tyr) in HEK293 cells.
Immunoprecipitation/Western Blot analysis of Phospho c-Met/HGFR (pan Tyr) in HeLa cells.
MILLIPLEX MAP Phospho c-Met/HGFR (panTyr) Magnetic Bead MAPmate™, 46-651MAG
Description:
MILLIPLEX MAP Phospho c-Met/HGFR (panTyr) Magnetic Bead MAPmate™, 46-651MAG
Trade Name:
MILLIPLEX
Qty/Pk:
1 kit
Product Overview:
The MILLIPLEX® MAP Phospho c-Met/HGFR (pan Tyr) Magnetic Bead MAPmate™ pair is used in conjunction with the MILLIPLEX® MAP Cell Signaling Buffer and Detection Kit (Catalog # 48-602MAG) to detect the presence of Phospho c-Met/HGFR (pan Tyr) in cell lysates using the Luminex 100™ IS, 200™, HTS, FLEXMAP 3D®, MAGPIX® system. Each MAPmate™ pair is ordered individually and may be combined for simultaneous multiplex analysis of cellular events. The MILLIPLEX® MAP Cell Signaling Buffer and Detection Kit is ordered separately and consists of a common set of reagents needed for performing MAPmate™ assays. The detection assay is a rapid, convenient alternative to Western Blotting and immunoprecipitation procedures. Each kit contains sufficient reagents for 100 individual assays. The MILLIPLEX® MAP HeLa Cell Lysate: Lambda Phosphatase included with this MAPmate™ may be utilized as an unstimulated control for this target, while the HEK293 Cell Lysate: Serum included with this MAPmate™ may be utilized as a stimulated control.
Important note: For a detailed protocol on Cell Signaling Detection Procedures please see the instruction booklet for the MILLIPLEX® MAP Cell Signaling Buffer and Detection Kit (Catalog # 48-602MAG).
Important note: For a detailed protocol on Cell Signaling Detection Procedures please see the instruction booklet for the MILLIPLEX® MAP Cell Signaling Buffer and Detection Kit (Catalog # 48-602MAG).
Key Applications:
Multiplexing
Application Notes:
• An overnight (4°C) incubation is recommended for best results
• This assay requires 25 µL diluted cell lysate per well
• Please consult the buffer compatibility chart or protocol for appropriate buffer choice
• 1 - 25 μg cell lysate/well (recommended starting concentration is 20 μg)
• This assay requires 25 µL diluted cell lysate per well
• Please consult the buffer compatibility chart or protocol for appropriate buffer choice
• 1 - 25 μg cell lysate/well (recommended starting concentration is 20 μg)
Species Reactivity:
Human
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Configuration:
Single Plex
UniProt Number:
Panel Type:
Cell Signaling
Storage Conditions:
Recommended storage for kit components is 2 - 8°C
Detection Methods:
Luminex xMAP
Product Name:
MILLIPLEX MAP Phospho c-Met/HGFR (panTyr) Magnetic Bead MAPmate™, 46-651MAG
Entrez Gene Number:
Materials Required but Not Delivered:
• MILLIPLEX MAP Cell Signaling Buffer and Detection Kit (Catalog #48-602MAG)
Analytes Available:
c-Met/HGFR (panTyr)