MILLIPLEX MAP Phospho STAT1 (Tyr701) MAPmate - Cell Signaling Single Plex or Multiplex Assay

MILLIPLEX MAP Phospho STAT1 (Tyr701) MAPmate

MILLIPLEX

1 kit

This MILLIPLEX® MAP Phospho STAT1 (Tyr701) MAPmate is a Cell Signaling single plex or multiplex assay. The analyte available for this kit is STAT1 (Tyr701).

Multiplexing

The MILLIPLEX MAP Phospho STAT1 (Tyr701) MAPmates pair is used in conjunction with the MILLIPLEX MAP Cell Signaling Buffer and Detection Kit (Catalog # 48-602) to detect the presence of phosphorylated STAT1 (Tyr701) in cell lysates using the Luminex® 100™ IS, 200™, or HTS system.

Human

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated by, and mediates the responses of many cell ligands, such as IL2, IL3, IL7, GM-CSF, erythropoietin, thrombopoietin, and different growth hormones. Activation of this protein in myeloma and lymphoma associated with a TEL/JAK2 gene fusion is independent of cell stimulus and has been shown to be essential for the tumorigenesis. The mouse counterpart of this gene is found to induce the expression of BCL2L1/BCL-X(L), which suggests the antiapoptotic function of this gene in cells.

Signal transducer and activator of transcription that mediates signaling by interferons (IFNs). Following type I IFN (IFN-alpha and IFN-beta) binding to cell surface receptors, Jak kinases (TYK2 and JAK1) are activated, leading to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize, associate with ISGF3G/IRF-9 to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of interferon stimulated genes, which drive the cell in an antiviral state. In response to type II IFN (IFN-gamma), STAT1 is tyrosine- and serine-phosphorylated. It then forms a homodimer termed IFN-gamma-activated factor (GAF), migrates into the nucleus and binds to the IFN gamma activated sequence (GAS) to drive the expression of the target genes, inducing a cellular antiviral state

Upstate

Recommended storage for kit components is 2 - 8°C

Phosphorylation

MILLIPLEX MAP Phospho STAT1 (Tyr701) MAPmate

IκBα, STAT1 (Tyr701)

Single Plex

• Anti-phospho STAT1 (Tyr701) Beads
• Anti- phospho STAT1 (Tyr701), biotin
• A431 Cell Lysate: EGF stimulated

P42224

Cell Signaling

• Fluorescent
• Luminex xMAP

NM_003152