Phospho-ZAP-70 Beadmates™
Description:
Phospho-ZAP-70 Beadmates™
Trade Name:
MILLIPLEX
Product Overview:
The Beadlyte® Phospho-ZAP-70 Beadmate™ pair is the optimal antibody pair for detection of phosphorylated ZAP-70. Beadmate™ antibody pairs are used in conjunction with the Beadlyte® Cell Signaling Buffer Kit (Catalog # 48-600, or 48-601) to detect the presence of phosphorylated ZAP-70 in cell lysates using the Luminex® xMAP™ system. Each Beadmate™ pair is ordered individually and can be combined for simultaneous multiplex analysis of cellular events. The Beadlyte® Cell Signaling Buffer Kit is also ordered separately and consists of a common set of reagents needed for using Beadmates™. The detection assay is a rapid, convenient alternative to Western Blotting and immunoprecipitation procedures. Each kit contains sufficient reagents for 100 individual assays.
Key Applications:
Multiplexing
Application Notes:
Important note: For a detailed protocol on Cell Signaling Detection Procedures please see the Certificate of Analysis for the Beadlyte® Cell Signaling Buffer Kit (select the highest lot number); available at:
Beadlyte® Cell Signaling Buffer Kit (Catalogue No. 48-600)
Beadlyte® Cell Signaling Buffer Kit (Catalogue No. 48-600)
Species Reactivity:
Human
Species Reactivity Note:
Recognizes phosphorylated human ZAP-70.
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Descriptive Text:
ZAP-70 is a non-receptor tyrosine kinase of the Syk family. It is a key early signaling mediator in T-cell activation and is normally only expressed in T-cells; expression in B-cells is a marker for poor prognosis in chronic lymphocytic leukemia. ZAP-70 becomes activated following ligation of the T-cell Receptor. It has two n-terminal SH2 domains, which bind ITAM motifs. When ZAP-70 is bound to an ITAM, Lck trans-phosphorylates and remains associated with it. Downstream effectors of the ZAP-70/Lck complex include LAT, Phospholipase Cγ, Vav, and the Rho and Ras GTPases. Autophosphorylation of ZAP-70 on Tyr319 is critical for association with Lck and thus for activation of downstream effectors.
Therapeutic Areas:
Oncology
Packaging:
Kit capacity: 100 assay points
Materials Required but Not Delivered:
• Cell lysates or cell extracts harboring protein(s) of interest
• Vortex mixer
• Plate shaker
• Timer
• Variable volume (5-200µl) pipette + tips
• Sonication Bath (Catalog # 40-002)
• Millipore multiscreen vacuum manifold (Catalog # MAVM0960R)
• Luminex® 100™ or Luminex® 200™ System
• Beadlyte® Cell Signaling Buffer Kit (Catalog # 48-600) or Cell Signaling Universal Buffer Kit (Catalog # 48-601)
• Vortex mixer
• Plate shaker
• Timer
• Variable volume (5-200µl) pipette + tips
• Sonication Bath (Catalog # 40-002)
• Millipore multiscreen vacuum manifold (Catalog # MAVM0960R)
• Luminex® 100™ or Luminex® 200™ System
• Beadlyte® Cell Signaling Buffer Kit (Catalog # 48-600) or Cell Signaling Universal Buffer Kit (Catalog # 48-601)
Kit Type:
- Cancer Kits
- Cell Signaling Kits
Components:
- Beadlyte® Anti-ZAP-70 Beads; One vial of anti-ZAP-70 IgG conjugated to Luminex® Bead # 51 / 125µL at 4,000 beads/µL (20X).
- Beadlyte® Anti-Phospho-ZAP-70, Biotin; One vial of biotin conjugated anti-phospho-ZAP-70 IgG / 125µL (20X).
Gene Symbol:
- ZAP70
- ZAP-70
- TZK
- STD
- SRK
Analytes Available:
ZAP-70
Detection Methods:
Luminex xMAP
Kit or Assay Type:
- Cancer Kits
- Cell Signaling Kits
- Multiplex Assays
Assay Type:
Multiplex Assays
Storage Conditions:
Stable for 1 year at 4°C from date of shipment. Store in the dark.