Ordering Information

Catalogue Number : 46-661
Qty/Pk : 1 kit

Product Family Information

	MILLIPLEX MAP – Human Cell Signaling Portfolio The cell’s ability to respond to its environment is essential for normal development, cellular repair and immunity and is the basis of homeostasis. Errors in these pathways, caused by mutations that may result in constitutive expression or total inhibition of protein transcription, are responsible for such diseases as diabetes, autoimmune disorders and cancer. » More…

MILLIPLEX MAP Phospho ZAP-70 MAPmate - Cell Signaling Single Plex or Multiplex Assay

Description:

MILLIPLEX MAP Phospho ZAP-70 MAPmate

Trade Name:

MILLIPLEX

Qty/Pk:

1 kit

Product Overview:

The Beadlyte® Phospho-ZAP-70 Beadmate™ pair is the optimal antibody pair for detection of phosphorylated ZAP-70. Beadmate™ antibody pairs are used in conjunction with the Beadlyte® Cell Signaling Buffer Kit (Catalog # 48-600, or 48-601) to detect the presence of phosphorylated ZAP-70 in cell lysates using the Luminex® xMAP™ system. Each Beadmate™ pair is ordered individually and can be combined for simultaneous multiplex analysis of cellular events. The Beadlyte® Cell Signaling Buffer Kit is also ordered separately and consists of a common set of reagents needed for using Beadmates™. The detection assay is a rapid, convenient alternative to Western Blotting and immunoprecipitation procedures. Each kit contains sufficient reagents for 100 individual assays.

Applications:

This MILLIPLEX® MAP Phospho ZAP-70 MAPmate is a Cell Signaling single plex or multiplex assay. The analyte available for this kit is ZAP-70 (pan Tyr).

Key Applications:

Multiplexing

Application Notes:

The MILLIPLEX MAP Phospho ZAP-70 MAPmates pair is used in conjunction with the MILLIPLEX MAP Cell Signaling Buffer and Detection Kit (Catalog # 48-602) to detect the presence of phosphorylated ZAP-70 in cell lysates using the Luminex® 100 IS, 200, or HTS system.

Species Reactivity:

Human

Species Reactivity Note:

Recognizes phosphorylated human ZAP-70.

Usage Statement:

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Entrez Gene Summary:

This gene encodes an enzyme belonging to the protein tyrosine kinase family, and it plays a role in T-cell development and lymphocyte activation. This enzyme, which is phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation, functions in the initial step of TCR-mediated signal transduction in combination with the Src family kinases, Lck and Fyn. This enzyme is also essential for thymocyte development. Mutations in this gene cause selective T-cell defect, a severe combined immunodeficiency disease characterized by a selective absence of CD8-positive T-cells. Two transcript variants that encode different isoforms have been found for this gene.

UniProt Summary:

FUNCTION: SwissProt: P43403 # Associates with the T-cell antigen receptor zeta chain (CD3Z). Plays a role in lymphocyte activation.
SIZE: 619 amino acids; 69872 Da
SUBUNIT: Interacts with SLA2 when it is phosphorylated. Interacts with CD3Z and with phosphorylated NFAM1. Interacts with CBLB (By similarity). Interacts with CBL and SLA when it is phosphorylated. The association with SLA (or SLA2) and CBL probably leads to its destruction. Interacts with SHB. Interacts with DEF6 (By similarity).
TISSUE SPECIFICITY: Expressed in T- and natural killer cells.
DOMAIN: SwissProt: P43403 The SH2 domain binds to the phosphorylated tyrosine-based activation motif (TAM) of CD3Z.
PTM: Phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation. Tyr-319 phosphorylation is essential for full activity.
DISEASE:SwissProt: P43403 # Defects in ZAP70 are the cause of selective T-cell defect (STD) [MIM:176947]. STD is an autosomal recessive form of severe combined immunodeficiency characterized by a selective absence of CD8-type T-cells.
SIMILARITY: Belongs to the protein kinase superfamily. Tyr protein kinase family. SYK/ZAP-70 subfamily. & Contains 1 protein kinase domain. & Contains 2 SH2 domains.

Brand Family:

Upstate

Storage Conditions:

Stable for 1 year at 4°C from date of shipment. Store in the dark.

Gene Symbol:

ZAP70
ZAP-70
TZK
STD
SRK

Product Name:

MILLIPLEX MAP Phospho ZAP-70 MAPmate

Materials Required but Not Delivered:

• Cell lysates or cell extracts harboring protein(s) of interest
• Vortex mixer
• Plate shaker
• Timer
• Variable volume (5-200µl) pipette + tips
• Sonication Bath (Catalog # 40-002)
• Millipore multiscreen vacuum manifold (Catalog # MAVM0960R)
• Luminex® 100™ or Luminex® 200™ System
• Beadlyte® Cell Signaling Buffer Kit (Catalog # 48-600) or Cell Signaling Universal Buffer Kit (Catalog # 48-601)

Analytes Available:

ZAP-70, ZAP-70 (pan Tyr)

Configuration:

Single Plex

Components: