MILLIPLEX MAP Total Akt/PKB MAPmate Assay - Cell Signaling Single Plex or Multiplex Assay
Description:
MILLIPLEX MAP Total Akt/PKB MAPmate, Assay Buffer 2
Trade Name:
MILLIPLEX
Qty/Pk:
1 kit
Applications:
This MILLIPLEX® MAP Total Akt/PKB MAPmate is a Cell Signaling single plex or multiplex assay. The analyte available for this kit is Akt/PKB (Total) - AB2.
Key Applications:
Multiplexing
Application Notes:
The MILLIPLEX MAP Total Akt/PKB MAPmates, Assay Buffer2, pair is used in conjunction with the MILLIPLEX MAP Cell Signaling Buffer and Detection Kit (Catalog # 48-602) to detect the presence of total Akt/PKB in cell lysates using the Luminex® 100 IS, 200, or HTS system.
Species Reactivity:
Human
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Entrez Gene Summary:
The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Multiple alternatively spliced transcript variants have been found for this gene.
UniProt Summary:
FUNCTION: SwissProt: P31749 # General protein kinase capable of phosphorylating several known proteins. Phosphorylates TBC1D4. Signals downstream of phosphatidylinositol 3-kinase (PI(3)K) to mediate the effects of various growth factors such as platelet-derived growth factor (PDGF), epidermal growth factor (EGF), insulin and insulin-like growth factor I (IGF-I). Plays a role in glucose transport by mediating insulin-induced translocation of the GLUT4 glucose transporter to the cell surface. Mediates the antiapoptotic effects of IGF-I. Mediates insulin-stimulated protein synthesis, partly by playing a role in both insulin-induced phosphorylation of 4E-BP1 and in insulin-induced activation of p70 S6 kinase. Promotes glycogen synthesis by mediating the insulin-induced activation of glycogen synthase.
SIZE: 480 amino acids; 55686 Da
SUBUNIT: Interacts with CENTG1 isoform 2 (PIKE-A) in the presence of guanine nucleotides. The C-terminus interacts with CCDC88A/GRDN. Interacts with AKTIP.
SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Note=Nucleus after activation by integrin-linked protein kinase 1 (ILK1).
TISSUE SPECIFICITY: In all human cell types so far analyzed.
DOMAIN: SwissProt: P31749 Binding of the PH domain to the phosphatidylinositol 3- kinase alpha (PI(3)K) results in its targeting to the plasma membrane.
PTM: Phosphorylation on Thr-308, Ser-473 and Tyr-474 is required for full activity. Ser-473 phosphorylation by the Rictor-mTor complex favors Thr-308 phosphorylation by PDPK1. Ser-473 phosphorylation is enhanced by interaction with CENTG1 isoform 2 (PIKE-A).
SIMILARITY: Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. RAC subfamily. & Contains 1 AGC-kinase C-terminal domain. & Contains 1 PH domain. & Contains 1 protein kinase domain.
SIZE: 480 amino acids; 55686 Da
SUBUNIT: Interacts with CENTG1 isoform 2 (PIKE-A) in the presence of guanine nucleotides. The C-terminus interacts with CCDC88A/GRDN. Interacts with AKTIP.
SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Note=Nucleus after activation by integrin-linked protein kinase 1 (ILK1).
TISSUE SPECIFICITY: In all human cell types so far analyzed.
DOMAIN: SwissProt: P31749 Binding of the PH domain to the phosphatidylinositol 3- kinase alpha (PI(3)K) results in its targeting to the plasma membrane.
PTM: Phosphorylation on Thr-308, Ser-473 and Tyr-474 is required for full activity. Ser-473 phosphorylation by the Rictor-mTor complex favors Thr-308 phosphorylation by PDPK1. Ser-473 phosphorylation is enhanced by interaction with CENTG1 isoform 2 (PIKE-A).
SIMILARITY: Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. RAC subfamily. & Contains 1 AGC-kinase C-terminal domain. & Contains 1 PH domain. & Contains 1 protein kinase domain.
Brand Family:
Upstate
Storage Conditions:
Recommended storage for kit components is 2 - 8°C.
Gene Symbol:
- AKT
- PKB
- C-AKT
- RAC-PK-alpha
- RAC-ALPHA
- PRKBA
- MGC99656
- RAC
- AKT1
Product Name:
MILLIPLEX MAP Total Akt/PKB MAPmate, Assay Buffer 2
Materials Required but Not Delivered:
MILLIPLEX MAP Cell Signaling Buffer and Detection Kit (Catalog # 48-602) Contains necessary reagents and instructions for sample, control, bead and detection antibody preparation
Protease inhibitors (Millipore Catalog #20-201 or equivalent)
Protease inhibitors (Millipore Catalog #20-201 or equivalent)
Analytes Available:
Akt (PKB), Akt/PKB (Total) - AB2
Components:
- Anti-Akt/PKB Biotin, Assay Buffer 2
- Anti-Akt/PKB Beads, Assay Buffer 2
UniProt Number:
Panel Type:
Cell Signaling
Detection Methods:
- Fluorescent
- Luminex xMAP
Packaging:
96-well Plate
Entrez Gene Number: