MILLIPLEX MAP Phospho-PTEN (Ser380) MAPmate - Cell Signaling Multiplex Assay
Description:
MILLIPLEX MAP Phospho-PTEN (Ser380) MAPmate
Trade Name:
MILLIPLEX
Qty/Pk:
1 kit
Applications:
This MILLIPLEX® MAP Phospho-PTEN (Ser380) MAPmate is a Cell Signaling single plex or multiplex assay. The analyte available for this kit is PTEN (Ser380).
Key Applications:
Multiplexing
Application Notes:
The MILLIPLEX MAP Phospho PTEN (Ser380) MAPmates pair is used in conjunction with the MILLIPLEX MAP Cell Signaling Buffer and Detection Kit (Catalog # 48-602) to detect the presence of phosphorylated PTEN (Ser380) in cell lysates using the Luminex® 100™ IS, 200™, or HTS system. Each MAPmates pair is ordered individually and may be combined for simultaneous multiplex analysis of cellular events. The MILLIPLEX MAP Cell Signaling Buffer and Detection Kit is ordered separately and consists of a common set of reagents needed for performing MAPmates assays. The detection assay is a rapid, convenient alternative to Western Blotting and immunoprecipitation procedures. Each kit contains sufficient reagents for 100 individual assays. The MILLIPLEX MAP HeLa Cell Lysate: Lambda Phosphatase treated included with this MAPmates pair may be utilized as a negative control for this target, while the HepG2 Cell Lysate: Insulin Stimulated included with this MAPmates pair may be utilized as a positive control.
Species Reactivity:
Human
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage Conditions:
Recommended storage for kit components is 2 - 8°C
Modifications:
Phosphorylation
Product Name:
MILLIPLEX MAP Phospho-PTEN (Ser380) MAPmate
Materials Required but Not Delivered:
• MILLIPLEX MAP Cell Signaling Buffer and Detection Kit (Catalog #48-602)
• Cell lysates or cell extracts harboring protein(s) of interest prepared using the MILLIPLEX MAP Cell Signaling Buffer and Detection Kit (Catalog # 48-602)
• Protease inhibitors (Millipore Catalog #20-201 or equivalent)
• Luminex Sheath Fluid (Luminex Catalog #40-50000)
• Cell lysates or cell extracts harboring protein(s) of interest prepared using the MILLIPLEX MAP Cell Signaling Buffer and Detection Kit (Catalog # 48-602)
• Protease inhibitors (Millipore Catalog #20-201 or equivalent)
• Luminex Sheath Fluid (Luminex Catalog #40-50000)
Analytes Available:
PTEN (Ser380)
Configuration:
Single Plex
Components:
- Anti-PTEN Beads
- Anti-phospho PTEN (Ser380), biotin
- MILLIPLEX MAP HeLa Cell Lysate: Lambda Phosphatase Treated
- MILLIPLEX MAP HepG2 Cell Lysate: Insulin Stimulated
UniProt Number:
Panel Type:
Cell Signaling
Detection Methods:
- Phosphoprotein
- Luminex xMAP
Entrez Gene Number: