MILLIPLEX MAP Human Phospho-Histone H2A.X (SER139) MAPmate

MILLIPLEX MAP Human Phospho-Histone H2A.X (SER139) MAPmate

MILLIPLEX

1 kit

Histone, H2A, H2B, H3, H4 are all involved in chromatin packing, organization, regulation and memory. One function of H2A.X is to signal the maintenance of a cll cycle arrest until repair is completed because it is rapidly phosphorylated at Ser¹³⁹ by the atazia-telangiectasia mutated (ATM) kinase in response to DNA double-strand breaks. The accumulation of high levels of H2A.X phosphorylation is a very early and accurate indicator of apoptosis.

Multiplexing

The MILLIPLEX MAP Phospho-Histone H2A.X (SER139) MAPmate pair is used in conjunction with the MILLIPLEX MAP Cell Signaling Buffer and Detection Kit (Catalog # 48-602) to detect the presence of phosphorylated Histone H2A.X (SER139) in cell lysates using the Luminex® 100 IS, 200, or HTS system.

Human

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a member of the histone H2A family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif

[FUNCTION] Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C-terminal phosphorylation.

Single Plex

• Anti-Histone H2A.X MILLIPLEX MAP
• Anti-Phosopho-Histone H2A.X (Ser139)

Upstate

P27661

Cell Signaling

• Fluorescent
• Luminex xMAP

100 points

MILLIPLEX MAP Human Phospho-Histone H2A.X (SER139) MAPmate

NM_002105

H2A.X (Ser139), β-Catenin