MILLIPLEX MAP Phospho BAD (Ser112) MAPmate - Cell Signaling Single Plex or Multiplex Assay

MILLIPLEX MAP Phospho BAD (Ser112) MAPmate

MILLIPLEX

1 kit

This MILLIPLEX® MAP Phospho BAD (Ser112) MAPmate is a Cell Signaling single plex or multiplex assay. The analyte available for this kit is BAD (Ser112).

Multiplexing

The MILLIPLEX MAP Phospho BAD (Ser112) MAPmates™ pair is used in conjunction with the MILLIPLEX MAP Cell Signaling Buffer and Detection Kit (Catalog # 48-602) to detect the presence of phosphorylated BAD (Ser112) in cell lysates using the Luminex® 100™ IS, 200™, or HTS system.

Human

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The protein encoded by this gene is a member of the BCL-2 family. BCL-2 family members are known to be regulators of programmed cell death. This protein positively regulates cell apoptosis by forming heterodimers with BCL-xL and BCL-2, and reversing their death repressor activity. Proapoptotic activity of this protein is regulated through its phosphorylation. Protein kinases AKT and MAP kinase, as well as protein phosphatase calcineurin were found to be involved in the regulation of this protein. Alternative splicing of this gene results in two transcript variants which encode the same isoform.

FUNCTION: SwissProt: Q92934 # Promotes cell death. Successfully competes for the binding to Bcl-X(L), Bcl-2 and Bcl-W, thereby affecting the level of heterodimerization of these proteins with BAX. Can reverse the death repressor activity of Bcl-X(L), but not that of Bcl-2 (By similarity). Appears to act as a link between growth factor receptor signaling and the apoptotic pathways.
SIZE: 168 amino acids; 18392 Da
SUBUNIT: Forms heterodimers with the anti-apoptotic proteins, Bcl- X(L), Bcl-2 and Bcl-W. Also binds protein S100A10 (By similarity). The Ser-75/Ser-99 phosphorylated form binds 14-3-3 proteins (By similarity).
SUBCELLULAR LOCATION: Mitochondrion outer membrane. Cytoplasm. Note=Upon phosphorylation, locates to the cytoplasm.
TISSUE SPECIFICITY: Expressed in a wide variety of tissues.
DOMAIN:SwissProt: Q92934 Intact BH3 motif is required by BIK, BID, BAK, BAD and BAX for their pro-apoptotic activity and for their interaction with anti-apoptotic members of the Bcl-2 family.
PTM: Phosphorylated on one or more of Ser-75, Ser-99, Ser-118 and Ser-134 in response to survival stimuli, which blocks its pro- apoptotic activity. Phosphorylation on Ser-99 or Ser-75 promotes heterodimerization with 14-3-3 proteins. This interaction then facilitates the phosphorylation at Ser-118, a site within the BH3 motif, leading to the release of Bcl-X(L) and the promotion of cell survival. Ser-99 is the major site of AKT/PKB phosphorylation, Ser-118 the major site of protein kinase A (CAPK) phosphorylation (By similarity).
SIMILARITY: Belongs to the Bcl-2 family.

Upstate

• BCL2L8
• BBC6
• BBC2
• BAD

MILLIPLEX MAP Phospho BAD (Ser112) MAPmate

BAD (Ser112)

Single Plex

• Anti-BAD Beads
• Anti-Phospho BAD (Ser112), biotin

Cell Signaling

• Luminex xMAP
• Fluorescent

100 points

• NM_032989.1
• NM_004322.2