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MILLIPLEX MAP Total BAD MAPmate - Cell Signaling Single Plex or Multiplex Assay
Description:
MILLIPLEX MAP Total BAD MAPmate
Trade Name:
MILLIPLEX
Qty/Pk:
1 kit
Product Overview:
Apoptosis, programmed cell death, is a tightly controlled process involved in both embryonic development and adult homeostasis in which the body is able to rid itself of damaged or unnecessary cells without inducing an inflammatory response. This essential process is triggered by both internal (DNA damage, mitochondrial dysfunction) and external (Fas and TNF receptor) signals. Dysregulated apoptosis that results in accelerated programmed cell death has been implicated in the development of various neurological diseases, such as Alzheimer’s and Parkinson’s disease, while inhibition of apoptosis is involved in the development of autoimmune disorders and cancer.
Applications:
This MILLIPLEX® MAP Total BAD MAPmate is a Cell Signaling single plex or multiplex assay. The analyte available for this kit is BAD (Total).
Key Applications:
Multiplexing
Application Notes:
The MILLIPLEX MAP Total BAD MAPmates pair is used in conjunction with the MILLIPLEX MAP Cell Signaling Buffer and Detection Kit (Catalog # 48-602) to detect the presence of total BAD in cell lysates using the Luminex® 100 IS, 200, or HTS system.
Species Reactivity:
Human
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Entrez Gene Summary:
The protein encoded by this gene is a member of the BCL-2 family. BCL-2 family members are known to be regulators of programmed cell death. This protein positively regulates cell apoptosis by forming heterodimers with BCL-xL and BCL-2, and reversing their death repressor activity. Proapoptotic activity of this protein is regulated through its phosphorylation. Protein kinases AKT and MAP kinase, as well as protein phosphatase calcineurin were found to be involved in the regulation of this protein. Alternative splicing of this gene results in two transcript variants which encode the same isoform.
UniProt Summary:
FUNCTION: SwissProt: Q92934 # Promotes cell death. Successfully competes for the binding to Bcl-X(L), Bcl-2 and Bcl-W, thereby affecting the level of heterodimerization of these proteins with BAX. Can reverse the death repressor activity of Bcl-X(L), but not that of Bcl-2 (By similarity). Appears to act as a link between growth factor receptor signaling and the apoptotic pathways.
SIZE: 168 amino acids; 18392 Da
SUBUNIT: Forms heterodimers with the anti-apoptotic proteins, Bcl- X(L), Bcl-2 and Bcl-W. Also binds protein S100A10 (By similarity). The Ser-75/Ser-99 phosphorylated form binds 14-3-3 proteins (By similarity).
SUBCELLULAR LOCATION: Mitochondrion outer membrane. Cytoplasm. Note=Upon phosphorylation, locates to the cytoplasm.
TISSUE SPECIFICITY: Expressed in a wide variety of tissues.
DOMAIN:SwissProt: Q92934 Intact BH3 motif is required by BIK, BID, BAK, BAD and BAX for their pro-apoptotic activity and for their interaction with anti-apoptotic members of the Bcl-2 family.
PTM: Phosphorylated on one or more of Ser-75, Ser-99, Ser-118 and Ser-134 in response to survival stimuli, which blocks its pro- apoptotic activity. Phosphorylation on Ser-99 or Ser-75 promotes heterodimerization with 14-3-3 proteins. This interaction then facilitates the phosphorylation at Ser-118, a site within the BH3 motif, leading to the release of Bcl-X(L) and the promotion of cell survival. Ser-99 is the major site of AKT/PKB phosphorylation, Ser-118 the major site of protein kinase A (CAPK) phosphorylation (By similarity).
SIMILARITY: Belongs to the Bcl-2 family.
SIZE: 168 amino acids; 18392 Da
SUBUNIT: Forms heterodimers with the anti-apoptotic proteins, Bcl- X(L), Bcl-2 and Bcl-W. Also binds protein S100A10 (By similarity). The Ser-75/Ser-99 phosphorylated form binds 14-3-3 proteins (By similarity).
SUBCELLULAR LOCATION: Mitochondrion outer membrane. Cytoplasm. Note=Upon phosphorylation, locates to the cytoplasm.
TISSUE SPECIFICITY: Expressed in a wide variety of tissues.
DOMAIN:SwissProt: Q92934 Intact BH3 motif is required by BIK, BID, BAK, BAD and BAX for their pro-apoptotic activity and for their interaction with anti-apoptotic members of the Bcl-2 family.
PTM: Phosphorylated on one or more of Ser-75, Ser-99, Ser-118 and Ser-134 in response to survival stimuli, which blocks its pro- apoptotic activity. Phosphorylation on Ser-99 or Ser-75 promotes heterodimerization with 14-3-3 proteins. This interaction then facilitates the phosphorylation at Ser-118, a site within the BH3 motif, leading to the release of Bcl-X(L) and the promotion of cell survival. Ser-99 is the major site of AKT/PKB phosphorylation, Ser-118 the major site of protein kinase A (CAPK) phosphorylation (By similarity).
SIMILARITY: Belongs to the Bcl-2 family.
Brand Family:
Upstate
Gene Symbol:
- BAD
- BBC2
- BBC6
- BCL2L8
Product Name:
MILLIPLEX MAP Total BAD MAPmate
Materials Required but Not Delivered:
Assay Buffer 1 (43-010)
Universal Lysis Buffer (43-040)
Beadlyte cell signaling streptavidin-phycoerythrin (45-0001D)
Milllipore 96-well filter plate (40-0008)
Universal Lysis Buffer (43-040)
Beadlyte cell signaling streptavidin-phycoerythrin (45-0001D)
Milllipore 96-well filter plate (40-0008)
Analytes Available:
BAD (Total), β-Catenin
Configuration:
Single Plex
Components:
- Anti-BAD Beads
- Anti-BAD, biotin
UniProt Number:
Panel Type:
Cell Signaling
Detection Methods:
- Fluorescent
- Luminex xMAP
Packaging:
100 points
Entrez Gene Number: