MILLIPLEX MAP Phospho NFĸB (Ser536) MAPmate - Cell Signaling Single Plex or Multiplex Assay
Description:
MILLIPLEX MAP Phospho NFĸB (Ser536) MAPmate, 46-702
Trade Name:
MILLIPLEX
Qty/Pk:
1 kit
Product Overview:
The MILLIPLEX MAP Phospho NFĸB (Ser536) MAPmates pair is used in conjunction with the MILLIPLEXMAP Cell Signaling Buffer and Detection Kit (Catalog # 48-602) to detect the presence of phosphorylated NFĸB (Ser536) in cell lysates using the Luminex® 100™ IS, 200™, or HTS system. Each MAPmates pair is ordered individually and may be combined for simultaneous multiplex analysis of cellular events. The MILLIPLEX MAP Cell Signaling Buffer and Detection Kit is ordered separately and consists of a common set of reagents needed for performing MAPmates assays. The detection assay is a rapid, convenient alternative to Western Blotting and immunoprecipitation procedures. Each kit contains sufficient reagents for 100 individual assays. The MILLIPLEX MAP HeLa Cell Lysate: Unstimulated included with this MAPmates pair may be utilized as a negative control for this target, while the HeLa Cell Lysate: TNFα + calyculin A included with this MAPmates pair may be utilized as a positive control.
Background Information:
Nuclear factor ĸB (NFĸB)
• Transcription factor
• In its Inactive form NFĸB is bound to IĸB in the cytosol
• Stimuli (cytokines, LPS, DNA damage, viral infections, etc.) lead to phosphorylation of IĸB, causing dissociation of NFĸB, which then enters the nucleus and acts as a transcription factor
• Involved in many biological processes, such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis.
• Transcription factor
• In its Inactive form NFĸB is bound to IĸB in the cytosol
• Stimuli (cytokines, LPS, DNA damage, viral infections, etc.) lead to phosphorylation of IĸB, causing dissociation of NFĸB, which then enters the nucleus and acts as a transcription factor
• Involved in many biological processes, such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis.
Applications:
This MILLIPLEX® MAP Phospho NFĸB (Ser536) MAPmate is a Cell Signaling single plex or multiplex assay. The analyte available for this kit is NFκB (Ser536).
Key Applications:
Multiplexing
Application Notes:
• This assay requires 25 μL diluted cell lysate (recommended starting concentration is 20 μg cell lysate/well)
• This is an overnight (4°C) or two-hour (RT) incubation assay; however, for best results an overnight incubation is recommended.
• MAPmate is compatible with both Assay Buffer 1(Catalog # 43-010) and Assay Buffer 2 (Catalog # 43-041)
• This is an overnight (4°C) or two-hour (RT) incubation assay; however, for best results an overnight incubation is recommended.
• MAPmate is compatible with both Assay Buffer 1(Catalog # 43-010) and Assay Buffer 2 (Catalog # 43-041)
Species Reactivity:
Human
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage Conditions:
Recommended storage for kit components is 2 - 8°C
Product Name:
MILLIPLEX MAP Phospho NFĸB (Ser536) MAPmate, 46-702
Materials Required but Not Delivered:
• MILLIPLEX MAP Cell Signaling Buffer and Detection Kit (Catalog #48-602)
• Cell lysates or cell extracts harboring protein(s) of interest prepared using the MILLIPLEX MAP Cell Signaling Buffer and Detection Kit (Catalog # 48-602)
• Protease inhibitors (Millipore Catalog #20-201 or equivalent)
• Luminex Sheath Fluid (Luminex Catalog #40-50000)
• Cell lysates or cell extracts harboring protein(s) of interest prepared using the MILLIPLEX MAP Cell Signaling Buffer and Detection Kit (Catalog # 48-602)
• Protease inhibitors (Millipore Catalog #20-201 or equivalent)
• Luminex Sheath Fluid (Luminex Catalog #40-50000)
Analytes Available:
NFκB (Ser536)
Configuration:
Single Plex
Components:
- MILLIPLEX MAP Anti-NFĸB Beads (20X)
- MILLIPLEX MAP Anti-phospho NFĸB (Ser536), biotin (20X)
- MILLIPLEX MAP HeLa Cell Lysate: Unstimulated
- MILLIPLEX MAP HeLa Cell Lysate: TNFα + calyculin A
UniProt Number:
Panel Type:
Cell Signaling
Detection Methods:
Luminex xMAP
Entrez Gene Number: