MILLIPLEX MAP Phospho Src (Tyr419) MAPmate - Cell Signaling Single Plex or Multiplex Assay
Description:
MILLIPLEX MAP Phospho Src (Tyr419) MAPmate, 46-710
Trade Name:
MILLIPLEX
Qty/Pk:
1 kit
Product Overview:
The MILLIPLEX MAP Phospho Src (Tyr419) MAPmates pair is used in conjunction with
the MILLIPLEX MAP Cell Signaling Buffer and Detection Kit (Catalog # 48-602) to detect
the presence of phosphorylated Src (Tyr419) in cell lysates using the Luminex® 100™
IS, 200™, or HTS system. Each MAPmates pair is ordered individually and may be
combined for simultaneous multiplex analysis of cellular events. The MILLIPLEX MAP
Cell Signaling Buffer and Detection Kit is ordered separately and consists of a common
set of reagents needed for performing MAPmates assays. The detection assay is a
rapid, convenient alternative to Western Blotting and immunoprecipitation procedures.
Each kit contains sufficient reagents for 100 individual assays. The MILLIPLEX
MAP HeLa Cell Lysate: Unstimulated included in the MILLIPLEX MAP Cell Signaling Buffer
and Detection Kit may be utilized as a negative control for this target, while the HeLa
Cell Lysate: Pervanadate included with this MAPmates pair may be utilized as a positive
control.
the MILLIPLEX MAP Cell Signaling Buffer and Detection Kit (Catalog # 48-602) to detect
the presence of phosphorylated Src (Tyr419) in cell lysates using the Luminex® 100™
IS, 200™, or HTS system. Each MAPmates pair is ordered individually and may be
combined for simultaneous multiplex analysis of cellular events. The MILLIPLEX MAP
Cell Signaling Buffer and Detection Kit is ordered separately and consists of a common
set of reagents needed for performing MAPmates assays. The detection assay is a
rapid, convenient alternative to Western Blotting and immunoprecipitation procedures.
Each kit contains sufficient reagents for 100 individual assays. The MILLIPLEX
MAP HeLa Cell Lysate: Unstimulated included in the MILLIPLEX MAP Cell Signaling Buffer
and Detection Kit may be utilized as a negative control for this target, while the HeLa
Cell Lysate: Pervanadate included with this MAPmates pair may be utilized as a positive
control.
Background Information:
Src:
• Proto-oncogene
• Nonreceptor Tyrosine Kinase
• Src Family Kinase members include Src, Yes, Fyn, Fgr, Lck, Hck, Blk, and Lyn
• Autophosphorylation of Tyr419 (activation site) and dephosphorylation of Tyr530 (inhibitory site) required to switch the kinase from the inactive closed formation to the active open formation
• High levels of Src found in platelets, neural tissue and osteoclasts.
• Mutations in this gene possibly involved in the malignant progression of colon cancer
• Important for proliferation, adhesion, differentiation, migration and survival through activation of Ras/ERK, Akt, and STAT pathways
• Proto-oncogene
• Nonreceptor Tyrosine Kinase
• Src Family Kinase members include Src, Yes, Fyn, Fgr, Lck, Hck, Blk, and Lyn
• Autophosphorylation of Tyr419 (activation site) and dephosphorylation of Tyr530 (inhibitory site) required to switch the kinase from the inactive closed formation to the active open formation
• High levels of Src found in platelets, neural tissue and osteoclasts.
• Mutations in this gene possibly involved in the malignant progression of colon cancer
• Important for proliferation, adhesion, differentiation, migration and survival through activation of Ras/ERK, Akt, and STAT pathways
Applications:
This MILLIPLEX® MAP Phospho Src (Tyr419) MAPmate is a Cell Signaling single plex or multiplex assay. The analyte available for this kit is Src (Tyr419).
Key Applications:
Multiplexing
Application Notes:
• This assay requires 25 μL diluted cell lysate (recommended starting concentration is 20 μg cell lysate/well)
• This is an overnight (4°C) or two-hour (RT) incubation assay; however, for best results an overnight incubation is recommended.
• MAPmate is compatible with both Assay Buffer 1(Catalog # 43-010) and Assay Buffer 2 (Catalog # 43-041)
• This is an overnight (4°C) or two-hour (RT) incubation assay; however, for best results an overnight incubation is recommended.
• MAPmate is compatible with both Assay Buffer 1(Catalog # 43-010) and Assay Buffer 2 (Catalog # 43-041)
Species Reactivity:
- Human
- Mouse
- Rat
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage Conditions:
Recommended storage for kit components is 2 - 8°C
Product Name:
MILLIPLEX MAP Phospho Src (Tyr419) MAPmate, 46-710
Materials Required but Not Delivered:
• MILLIPLEX MAP Cell Signaling Buffer and Detection Kit (Catalog #48-602)
• Cell lysates or cell extracts harboring protein(s) of interest prepared using the MILLIPLEX MAP Cell Signaling Buffer and Detection Kit (Catalog # 48-602)
• Protease inhibitors (Millipore Catalog #20-201 or equivalent)
• Luminex Sheath Fluid (Luminex Catalog #40-50000)
• Cell lysates or cell extracts harboring protein(s) of interest prepared using the MILLIPLEX MAP Cell Signaling Buffer and Detection Kit (Catalog # 48-602)
• Protease inhibitors (Millipore Catalog #20-201 or equivalent)
• Luminex Sheath Fluid (Luminex Catalog #40-50000)
Analytes Available:
Src (Tyr419)
Configuration:
Single Plex
Components:
- MILLIPLEX MAP Anti-Src Beads (20X)
- MILLIPLEX MAP Anti--phospho Src (Tyr419), biotin (20x)
- MILLIPLEX MAP HeLa Cell Lysate: Pervanadate
UniProt Number:
Panel Type:
Cell Signaling
Detection Methods:
Luminex xMAP
Entrez Gene Number: